Anti CD40 monoclonal antibody [Clone 5C3]


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Background

CD40 is a 45-50-kDa glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. CD40 is specifically expressed on the surface of B cells and specialized antigen-presenting cells such as dendritic cells and macrophages. CD40 interacts with the CD40 ligand (CD154) which is found primarily on T cells, playing a role in both humoral and cell-mediated immune responses. Activation of CD40 on B cells by CD40 ligand causes B cell proliferation, differentiation, immunoglobulin isotype switching, germinal center formation, and stimulation of the humoral memory response. CD40 has been found to mediate a broad variety of immune and inflammatory responses. Within the cell, the CD40 molecule acts as a transmembrane signal transducer that leads to activation of intracellular kinases and transcription factors.
The antibody against human CD40 was produced from hybridoma (5C3) cultured in serum-free medium and the IgG was purified under mild conditions by propriety chromatography processes.

Data Link : Swiss-Prot P25942

Application

1) Flowcytometry
2) Immunohistochemistry
3) Stimulation of B cell proliferation in the presence of IL4.

B cell-specific expression of h (human) CD40 in transgenic mice (ref.2).
Splenocytes from m (mouse) CD40+/-, mCD40-/- and hCD40 wild type/mCD40-/- mice were stained with monoclonal antibodies against mCD40, B220 and hCD40 (5C3) and analyzed by flow cytometry. hCD40 molecules were expressed specifically on B220+ B cells.

<Reference>
1. Inui S et al (1990) “Identification of the intracytoplasmic region essential for signal transduction through a B cell activation molecule, CD40.” Eur J Immunol 20: 1747-1753 PMID: 1698631
2. Yasui T et al (2002) “Dissection of B cell differentiation during primary immune responses in mice with altered CD40 signals.” Int Immunol 14: 319-329 PMID: 11867568
3. Ishida I et al (2003) “Involvement of CD100, a lymphocyte semaphoring, in the activation of the human immune system via CD72: implications for the regulation of immune and inflammatory responses.” Int Immunol. 15: 1027-1034 PMID: 12882840

Product List

Product Name Cat# Quantity Price

Anti CD40 (Mouse)  DataSheet

BAM-72-030-EX

100 UG ¥31,500
$420

Anti CD40 (Mouse) Biotin DataSheet

BAM-72-031-EX

50 UG ¥31,500
$420

Anti CD40 (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-72-032-EX

50 UG ¥31,500
$420

Product Name Cat# Quantity Price

Anti Fc (ε) RI (α), Human (Mouse) Unlabeled DataSheet

BAM-72-001-EX

100 UG ¥34,500
$460

Anti Fc (ε) RI (α), Human (Mouse) Biotin DataSheet

BAM-72-003-EX

50 UG ¥34,500
$460

Anti Fc (ε) RI (α), Human (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-72-004-EX

50 UG ¥34,500
$460

Anti Fc (ε) RI (α), Human (Mouse) Unlabeled DataSheet

BAM-72-005-EX

100 UG ¥34,500
$460

Anti Fc (ε) RI (α), Human (Mouse) Biotin DataSheet

BAM-72-007-EX

50 UG ¥33,000
$440

Anti Fc (ε) RI (α), Human (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-72-008-EX

50 UG ¥33,000
$440

Anti CD40 (Mouse)  DataSheet

BAM-72-030-EX

100 UG ¥31,500
$420

Anti CD40 (Mouse) Biotin DataSheet

BAM-72-031-EX

50 UG ¥31,500
$420

Anti CD40 (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-72-032-EX

50 UG ¥31,500
$420

“Anti CD40 monoclonal antibody [Clone 5C3]” also belongs to the following categories.