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  • Anti MAGE-D1 / Dlxin-1 / NRAGE [MD1] polyclonal antibody

useful for the research of Brain and Neuroscience.

Anti MAGE-D1 / Dlxin-1 / NRAGE [MD1] polyclonal antibody


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Background

MAGE-D1 (melanoma-associated antigen D1), also known as Dlxin-1 or NRAGE, is a member of the MAGE protein family. MAGE-D1 is expressed in almost all normal adult tissues and has been demonstrated to interact with the p75 neutrophin receptor and to facilitate nerve growth factor-dependent apoptosis (ref.1). MAGE-D1 also interacts with its homologous protein, necdin, which is known as a growth suppressor and a promoter of differentiation in neurons. Necdin binds to Msx (msh homeobox) and Dlx (distal-less homeobox) family homeodomain transcription factors via MAGE-D1. These proteins cooperate to modulate differentiation of neurons and muscle cells by regulating gene expression whereas Msx proteins function as transcriptional repressor and Dlx proteins function as transcriptional activators (ref.2 and 3).
An antibody (named MD1) against mouse MAGE-D1 was raised in rabbit (ref.2).

Data Link : Swiss-Prot  Q9QYH6 (mouse), Q9ES73 (rat), Q9Y5V3 (human)

Application

1) Western blotting (dilution: 1/3,000-1/1,000)
2) Immunohistochemistry (dilution: 1/300)
3) Immunocytochemistry (dilution: 1/1,000-1/500)

Not tested for other applications


<
Western blotting >
Western blot analysis of necdin and MAGE-D1 in ouse embryonal tissues (ref.2).
Tissue lysates (20ug) from E18.5 embryos were separated by SDS-PAGE and immunoblotted with antibodies against necdin, MAGE-D1 (D1) and tubulin.
Endogenous ~43-kDa necdin protein was detected almost exclusively in the brain and skeletal muscle whereas endogenous ~85-kDa MAGE-D1 protein was expressed in a ubiquitous manner.


< Immunohistochemistry >
Fluorescence immunohistochemistry for ecdin and MAGE-D1 in mouse embryos.
Adjacent sections of the forebrain at E12.5 embryo (upper panels) and the hind limb at E14.5 (lower panels) were stained with ntibodies against necdin and MAGE-D1.
The arrows point to the preplate (upper panels) and the skeletal muscles (lower panels). The arrowheads indicate the ventricular proliferative zone; V, ventricle; , C bone cavity.
Necdin and MAGE-D1 were concentrated in the preplate of the forebrain at E12.5 and skeletal muscle tissues in the hind limb at E14.5. In developing neural tube, MAGE-D1 immunoreactivity was distributed in the ventricular zone as well as the marginal zone.


< Immunocytochemistry >
C2C12 cells were cultured under undifferentiated conditions (UD) or under differentiation conditions for 4 days (4D). Cells were fixed and triply stained for myosin heavy chain (MHC), MAGE-D1 (D1), and nuclear DNA with Hoechst33342 (Hoechst). The arrowheads point to differentiated ultinucleated myocytes.
MHC and MAGE-D1 were distributed predominantly in the cytosol of multinucleated differentiated C2C12 cells.

<Reference>
1. Salehi AH et al (2000) “NRAGE, a novel MAGE protein, interacts with the p75 neutrophin receptor and facilitates nerve growth factor dependent apoptosis.” Neuron 27: 279-288 PMID: 10985348
2. Kuwajima T et al (2004) “Necdin interacts with the Msx2 homeodomain protein via MAGE-D1 to promote myogenic differentiation of C2C12 cells.” J Biol Chem 279: 40484-40493 PMID: 15272023
3. Kuwajima T et al (2006) “Necdin promotes GABAergic neuron differentiation in cooperation with Dlx homeodomain proteins.” J Neurosci 26: 5383-5392 PMID: 16707790

Product List

Product Name Cat# Quantity Price

Anti MAGE-D1 / Dlxin-1 / NRAGE, Mouse (Rabbit)  DataSheet

BAM-74-112-EX

100 UL ¥34,500
$460

Product Name Cat# Quantity Price

Anti Glyoxalase I, Mouse (Rat) Unlabeled DataSheet

BAM-74-001-EX

100 UG ¥34,500
$460

Anti Necdin, Mouse (Rabbit) Unlabeled DataSheet

BAM-74-100-EX

100 UL ¥34,500
$460

Anti Activated Caspase 3 (Rabbit)  DataSheet

BAM-74-102-EX

100 UL ¥34,500
$460

Anti APP, Human (Rabbit)  DataSheet

BAM-74-104-EX

100 UL ¥34,500
$460

Anti APP, Human (Rabbit)  DataSheet

BAM-74-106-EX

100 UL ¥34,500
$460

Anti APP-C31, Human (Rabbit)  DataSheet

BAM-74-108-EX

100 UL ¥34,500
$460

Anti APP (Δ) C31, Human (Rabbit)  DataSheet

BAM-74-110-EX

100 UL ¥34,500
$460

Anti MAGE-D1 / Dlxin-1 / NRAGE, Mouse (Rabbit)  DataSheet

BAM-74-112-EX

100 UL ¥34,500
$460

Anti MAGE-G1 / Necdin-like 2, Mouse (Rabbit)  DataSheet

BAM-74-114-EX

100 UL ¥34,500
$460

“Anti MAGE-D1 / Dlxin-1 / NRAGE [MD1] polyclonal antibody” also belongs to the following categories.