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Anti Rpn12 (S.cerevisiae) polyclonal antibody


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Background

The 26 S proteasome is a protein complex with a molecular massof 2,000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteinsinvolved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (ref.1). The 26S proteasome is composed of the 20S core particle (CP) and the 19S regulatory particle (RP). The RP is further subdivided into lid and base sub-complexes. Rpn12 is one of the non-ATPase subunits of the lid. Rpn12 interacts with an ATPase subunit, Rpt1, of the base. Rpn12, Rpt1 double mutant becomes lethal, suggesting a strong interaction between Rpn12 and Rpt1. In the double mutant cells, the function of the 26S proteasome is completely eliminated.

Data Link SGD RPN12/YFR052W

Application

1) Western blotting (1/5,000~1/10,000)
2) Immunoprecipitation

 


Western blotting.
Detection of Rpn12 (32kD) in the crude extract of S. cerevisiae

<Reference>
1. Hershko A and Ciechanover A “THE UBIQUITIN SYSTEM.” Annu. Rev. Biochem. 67, 425-479 (1998) PMID: 9759494
2. Takeuchi J and Toh-e A “Genetic evidence for interaction between components of the yeast 26S proteasome: combination of a mutation in RPN12 (a lid component gene) with mutations in RPT1 (an ATPase gene) causes synthetic lethality.” Mol Gen Genet 262:145-153 (1999) PMID: 10503546
3. Tone Y et al “Nob1p, a new essential protein, associates with the 26S proteasome of growing saccharomyces cerevisiae cells.” Gene 243: 37-45 (2000) PMID: 10675611

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