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Anti Tem1 (S.cerevisiae) polyclonal antibody


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Background

Tem1 is a low-molecular-weight GTP-binding protein (GTPase) which is required for the termination of M phase in cell division. The defect of Tem1 was lethal, and the Tem1-defective cells were arrested at telophase with high H1-kinase activity, indicating that Tem1 is required to exit from M phase. The defect of Tem1 was suppressed by a high dose of cdc15, which encodes a protein kinase. Tem1 functions upstream of cdc15 kinase and may be required to activate the cdc15 protein kinase pathway. A cascade consisting of Tem1 and kinases act to terminate mitosis.

 Data Link SGD TEM1/YML064C

Application

1) Western blotting (1/250~1/500)
2) Immunoprecipitation

 

Western blotting.
Detection of Tem1 (28kD) in the crude extract of S. cerevisiae 

<Reference>
1. Shirayama M et al “The yeast TEM1 gene, which encodes a GTP-binding protein, is involved in termination of M phase.” Mol Cell Biol 14, 7476-7482 (1994) PMID: 7935462
2. Shou W et al “Exit from mitosis is triggered by Tem1- dependent release of protein phosphatase Cdc14 from nucleolar RENT complex.” Cell 97:233-244 (1999) PMID: 10219244
3. Lippincott J et al “The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis.” J Cell Sci 114:1379-1386 (2001) PMID: 11257003

Product List

Product Name Cat# Quantity Price

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100 UL ¥42,000
$560

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