Anti p53 Phospho-Ser20 monoclonal antibody


print

Background

p53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene.  p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids.  In the unstressed normal cells, the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis.  The functions of p53 are regulated by  phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule (1).
p53 at Ser20 is phosphorylated by Chk1 and Chk2 kinases and this enhances tetramerization, stability and activity of p53 (2, 3).

Data Link  UniProtKB/Swiss-Prot P04637 (P53_HUMAN)

Application

1) Western blotting (-1 ug/ml)
2) ELISA
3) Other applications were not tested.


Identification of p53 phospho-Ser20 with monoclonal antibody 17B6 by Western blotting.
The lysates of H1299 cells (p53 non-producing) transfected either with Flag vector (left lane), Flag-p53 (wild-type) expressing plasmid (middle lane) or Flag-p53 mutant (Ser20Ala) expressing plasmid (right lane) were immunoprecipitated with anti-Flag antibody and then analyzed by Westernbotting with anti-p53 p-S20 monoclonal antibody, 17B6, (upper panel) or with pantropic p-53 antibody (lower panel).

WESTERN BLOTTING PROTOCOL (PDF) PDF

<Reference>
1. Bode AM & Dong Z “Post-translational modification of p53 in tumorigenesis”Nature Rev Cancer 4: 793 -805 (2004) PMID: 15510160
2. Shieh SY et al “DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization”EMBO J 18: 1815-1823 (1999) PMID: 10202145
3. Hirao A “DNA damage-induced activation of p53 by the checkpoint kinase Chk2” Science 287: 1824-1827 (2000) PMID: 10710310

Product List

Product Name Cat# Quantity Price

Anti p53, phospho Ser20, Human (Mouse) Unlabeled DataSheet

BAM-71-113-EX

50 UG see also
# BAM-71113EX
Size 100

Product Name Cat# Quantity Price

Anti RanBPM/BP9, Human (Rabbit) Unlabeled DataSheet

BAM-71-001-EX

50 UL see also
# BAM-71001EX
Size 100

Anti RanBPM/BP9, Human (Rabbit) Unlabeled DataSheet

BAM-71-002-EX

250 UL N/A

Anti RanBP2, Human (Rabbit) Unlabeled DataSheet

BAM-71-003-EX

50 UL see also
# BAM-71003EX
Size 100

Anti RanBP2, Human (Rabbit) Unlabeled DataSheet

BAM-71-004-EX

250 UL N/A

Anti p53, phospho Ser20, Human (Mouse) Unlabeled DataSheet

BAM-71-113-EX

50 UG see also
# BAM-71113EX
Size 100

Anti p53, phospho Ser46, Human (Mouse) Unlabeled DataSheet

BAM-71-115-EX

50 UG see also
# BAM-71115EX
Size 100

Anti p53, phospho Ser315, Human (Mouse) Unlabeled DataSheet

BAM-71-117-EX

50 UG see also
# BAM-71117EX
Size 100

Anti p53, acetyl Lys120, Human (Mouse) Unlabeled DataSheet

BAM-71-131-EX

50 UG see also
# BAM-71131EX
Size 100

Anti p53, acetyl Lys382, Human (Mouse) Unlabeled DataSheet

BAM-71-133-EX

50 UG see also
# BAM-71133EX
Size 100

Anti Mdmx/Hdmx, phospho Ser367, Human (Mouse) Unlabeled DataSheet

BAM-71-141-EX

50 UG see also
# BAM-71141EX
Size 100

Anti E2F1, Human (Mouse) Unlabeled DataSheet

BAM-71-151-EX

50 UG see also
# BAM-71151EX
Size 100

Anti c-Myc, Human (Mouse) Unlabeled DataSheet

BAM-71-161-EX

50 UG see also
# BAM-71161EX
Size 100

Anti RB, phospho Ser795, Human (Mouse) Unlabeled DataSheet

BAM-71-171-EX

50 UG see also
# BAM-71171EX
Size 100

Anti RB, phospho Thr821, Human (Mouse) Unlabeled DataSheet

BAM-71-173-EX

50 UG see also
# BAM-71173EX
Size 100

Anti KDM5A/RBP2/JARID1A, Human (Mouse) Unlabeled DataSheet

BAM-71-175-EX

50 UG see also
# BAM-71175EX
Size 100

Anti KDM5A/RBP2/JARID1A, Human (Mouse) Unlabeled DataSheet

BAM-71-177-EX

50 UG see also
# BAM-71177EX
Size 100

Anti EID1, Human (Mouse) Unlabeled DataSheet

BAM-71-185-EX

50 UG see also
# BAM-71185EX
Size 100

Anti EID1, Human (Mouse) Unlabeled DataSheet

BAM-71-190-EX

50 UG see also
# BAM-71190EX
Size 100

Anti PHLDA3, Human (Mouse)  DataSheet

BAM-71-195-EX

100 UG ¥34,500
$460

Anti HB-EGF, Human (Mouse) Unlabeled DataSheet

BAM-71-501-EX

50 UG see also
# BAM-71501EX
Size 100

Anti HB-EGF, Human (Mouse) Biotin DataSheet

BAM-71-503-EX

50 UG ¥34,500
$460

“Anti p53 Phospho-Ser20 monoclonal antibody” also belongs to the following categories.