Reagents for Genetic Engineerring

Pfu DNA Polymerase(+dNTPs), Economy


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Background

Pyrococcus furiosus DNA polymerase (Pfu DNA polymerase) gene was expressed in E.Coli in large quantities and highly purified. The enzyme has thermostable DNA polymerase activity and 3’ →5’ exonuclease (proofreading) activity. The MW is 90 kDa, same as that of the natural Pfu DNA polymerase.

¡ Pfu DNA polymerase is thermostabe and has low error rates.
¡ It is suitable for PCR and primer extension reactions that require high fidelity synthesis.
¡ Pfu DNA polymerase-generated PCR fragments are blunt-ended.

Application

1) cloning
2) DNA expression
3) site-directed mutagenesis

General composition of PCR reaction mixture (total 50ml)
Pfu DNA polymerase (2.5 units/ml) 0.5 ml
10 x Reaction Buffer (Pfu) 5 ml
2.5mM (each) dNTPs 4ml
Template <500ng
Primer 1 0.2`1.0mM (final conc.)
Primer 2 0.2`1.0mM (final conc.)
Sterile distilled water up to 50ml

 

< SDS-PAGE of Taq DNA polymerase >

< Amplification of λ DNA >

Product List

Product Name Cat# Quantity Price

Pfu DNA polymerase (+dNTPs), Economy DataSheet ,

BAM-02-021-EX

200 UNIT ¥8,300
$111

Pfu DNA polymerase (+dNTPs), Economy DataSheet ,

BAM-02-021-5EX

5*200 UNIT ¥33,200
$443

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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