Tag proteins antibody

Anti His6 Tag Polyclonal Antibody


print

Background

Epitope tagging has become a powerful tool for detection and purification of expressed proteins. His6-epitope tag is especially useful because the tagged recombinant proteins can be easily purified by affinity chromatography with metal ion (e.g.Ni2+, Co2+ etc.)) conjugated resins. Due to their small size, His epitope tag does not affect the tagged protein's biochemical properties in most cases.
Anti-His epitope tag antibody is useful for identification of a recombinant protein tagged with 6X His at N-terminal, C-terminal and internal of the proteins.

Application

1) Western blotting (dilution : 1/1,000 )
2) ELISA (Individual use)


Fig.1  Detection of His-tagged proteins (FLAG-His6-EmGFP, FLAG-His6-Em GFP-PRMT6) in crude extract of 293T cells expressing the recombinant proteins with anti-EGFP antibody (left panel,) and anti-His6 antibody (right panel).
The antibodies were used at x 1,000 fold dilution.

References

1. Simpson RJ, Adams PD and Golemis EA " Basic Methods in Protein Purification and Analysis.A laboratory Manual" ColdSpring Harbor Laboratory Press (2009) New York

Product List

Product Name Cat# Quantity Price

Anti His6 Tag (Rabbit)  DataSheet

BAM-60-051-EX

100 UL ¥22,000
$294

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Anti His6 Tag Polyclonal Antibody” also belongs to the following categories.