Tag proteins antibody

Anti GFP polyclonal antibody


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Background

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization (2, 3).

Data Link : Swiss-Prot P42212 (GFP_AEQVI)

Application

1) Western blotting (dilution : 1/2,000 )
2) Immunoprecipitation (Individual use)
3) ELISA

Fig.1 Detection of GFP protein by Western blotting.

- : Lysate of 293T cells transfected with an empty vector
+ : Lysate of 293T cells transfected with the plasmid carrying the GFP gene

 

Fig.2 Detection of GFP-tagged protein followed by Western blotting.

- : Lysate of 293T cells transfected with an empty vector
+ : Lysate of 293T cells transfected with the plasmid carrying the GFP-tagged NPM1 gene

<Reference>
1. Shimomura O et al (1962) "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea." J Cell Comp Physiol 59: 223–239 PMID: 13911999
2. Chalfie M et al (1994) "Green fluorescent protein as a marker for gene expression." Science 263 (5148):
 802–805 PMID: 8303295
3. Tsien R (1998) "The green fluorescent protein." (PDF) Annu Rev Biochem 67: 509–544 PMID: 9759496

Product List

Product Name Cat# Quantity Price

Anti GFP Aequorea victoria  (Rabbit) Unlabeled DataSheet

BAM-60-011-EX

100 UL ¥22,000
$294

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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