Anti DDX3X polyclonal antibody


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Background

DDX3X (662aa, 73.2 kDa) is a member of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD) which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. This gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog. Alternative splicing results in multiple transcript variants.

  UniProtKB/Swiss-Prot O00571 (DDX3X_HUMAN)
  GeneCards:DDX3X Gene

Application

1. Western blotting (dilution: 1/1,000-1/5,000)
2. Immunofluorescence staining (dilution : 1/500)

< Western blot analysis of DDX3 in the whole cell extracts HeLa cells (10mg) >

< Immunostaing of HeLa cells with anti-DDX3X antibody >
Paraformaldehyde-fixed HeLa cells were stained with anti-DDX3X antibody (green). DDX3X is localized in cytoplasm in the absence of treatment, but once treated with LMB, a nuclear translocation inhibitor, it is localized not only in cytoplasm but also in nuclei.

<Reference>
1. Sekiguchi T et al “Human DDX3Y, the Y-encoded isoform of RNA helicase DDX3, rescues a hamster temperature-sensitive ET24 mutant cell line with a DDX3X mutation." Exp. Cell. Res. 300 : 213-222 (2004) PMID: 15383328

Product List

Product Name Cat# Quantity Price

Anti DDX3X, Human (Rabbit) Unlabeled DataSheet

BAM-70-450-EX

100 UL ¥33,000
$440

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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