Anti DDX18 polyclonal antibody


print

Background

DDX18 protein (670aa, 75.4KDa) is a putative ATP-dependent RNA helicase since it possesses a DEAD box motif, characterized by the conserved motif, Asp-Glu-Ala-Asp (DEAD).RNA helicases which may play a role in ribosome biogenesis. DDX18 acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It was also involved in HIV-1 replication and interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location.

Application

Western blotting (1/1,000 – 1/2,000 fold dilution)
Immunofluorescent staining (1/500 fold dilution)

BAM_70453EX_1
Fig.1 Western blot analysis of DDX3 in the whole cell extracts of
HeLa cells (10mg) with anti -DDX18 antibody at 1,000 fold dilution

BAM_70453EX_2
Fig.2 Immunostaining of DDX18 in HeLa cells with anti DDX18 antibody 
followed by FITC-conjugated anti-rabbit IgG second antibody. DNA was 
stained with Hoechst dye.

References

Sekiguchi T et al gNOP132 is required for proper nucleolus localization of DEAD-box
RNA helicase DDX47." Nucleic. Acid. Res. 34 : 4593-4606 (2006) PMID: 16963496

Product List

Product Name Cat# Quantity Price

Anti DDX18, Human (Rabbit) Unlabeled DataSheet

BAM-70-453-EX

100 UL ¥33,000
$440

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



“Anti DDX18 polyclonal antibody ” also belongs to the following categories.