Anti-RuvC antibody, rabbit polyclonal


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Background

E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombinatioin repair and introduces nicks at the symmetrical points of the Holliday junction, cleaving and resolving the recombinant (1). Its molecular weight is 19kD and forms a dimmer in liquid.

Application

Western blotting: x 3,000 dilution

BAM_61009EX_1
Fig.1 Detection of RuvC (19kD) proteins in the cell extracts of 01-011 E.coli RuvC protein E. coli carrying the plasmids which encode the indicated mutant RuvC proteins by Western blotting using this antibody (Ref. 2).
WT: wild type RuvC protein
vector: vector plasmid
D7G, D7N, P8L: mutant RuvC proteins

References

1) Shinagawa H and Iwasaki H (1996) gProcessing the holliday junction in homologous recombinationh Trends
Biochem Sci 21:107-111 PMID: 8882584
2) Ichiyanagi K et al (1998) gMutational analysis on structure-function relationship of a holliday junction specific
endonuclease RuvCh Genes to Cells 3: 575-586 PMID: 9813108
3) Saito A et al (1995) gIdentification of four acidic amino acids that constitute the catalytic center of the RuvC
Holliday junction resolvaseh Proc Natl Acad Sci USA 92: 7470-7474 PMID: 7638215
4) Friedberg EC et al DNA Repair and Mutagenesis 2nd ed. ASM Press

Product List

Product Name Cat# Quantity Price

Anti RuvC Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-009-EX

100 UL ¥31,600
$422

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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