Anti-RuvA antibody, rabbit polyclonal


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Background

E. coli RuvA protein binds specifically to the Holliday structure which is the intermediate of recombination at the late stage of homologous recombination and recombination repair, and forms a complex with RuvB motor protein, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. In solution, it forms a tetramer and binds to the cross-like DNA of the Holliday junction from below and above, holding it in between (1, 2). Using this antiserum in Western blotting, the band of 22kD corresponding to RuvA was obtained from the extract of E. coli cells (Fig.1).

Application

Western blotting: x3,000 dilution (Fig. 1)
ELISA 

BAM_61005EX_1
Fig1. Detection of RuvA (22kD) protein by Western blotting using this antibody.
lane1: RuvA protein 0.8ng
lane2: E. coli AB1157 crude extract
lane3: E. coli AB1157 lexA mutant crude extract
Expression of RuvA is enhanced by lexA mutation.

References

1) Shinagawa H and Iwasaki H (1996) gProcessing the holliday junction in homologous recombinationh Trends
Biochem Sci 21:107-111PMID: 8882584
2) Iwasaki H et al (1992) gEscherichia coli RuvA and RuvB proteins specifically interact with Holliday junctions
and promote branch migrationh Genes Dev 6:2214-2220 PMID: 1427081

Product List

Product Name Cat# Quantity Price

Anti RuvA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-005-EX

100 UL ¥33,000
$440

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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