E. coli RuvC Protein


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Background

E. coli RuvC protein is a structurally specific endonuclease which binds specifically to the Holliday structure, an intermediate of recombination, at the late stage of homologous recombination and recombination repair and introduces a nick in the symmetrical point of the Holliday junction cleaving and resolving the recombinant (1, 2). The product is a recombinant protein abundantly expressed by E. coli and purified by methods such as chromatography (Fig. 1). Its molecular weight is 19 kD and forms a dimer in liquid and in binding tate with the Holliday structure.

Application

1) Studies on homologous recombination mechanism
2) To use as endonuclease which functions specifically to the Holliday structure

BAM_01011EX_1
Fig.1 Polyacrylamide gel electrophoresis of RuvC protein.

References

1. Shinagawa H and Iwasaki H (1996) gProcessing the holliday junction in homologous
recombination.h Trend Biochem Sci 21:107-111 PMID: 8882584
2. Iwasaki H et al (1991) gEscherichia coli RuvC protein is an endonuclease that resolves
the Holliday structure.h EMBO J 10:4381-4389 (1991) PMID: 1661673
3. Murayama Y et al (2008) gFormation and branch migration of Holliday junctions
mediated by eukaryotic recombinases.hNature 451:1018-1021PMID:18256600

Product List

Product Name Cat# Quantity Price

RuvC Protein Escherichia coli DataSheet

BAM-01-011-EX

20 UG ¥42,000
$560

RuvC Protein Escherichia coli DataSheet

BAM-01-012-EX

100 UG ¥126,000
$1680

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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