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E. coli Ribonuclease H (RNase H), Recombinant


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Background

Ribonuclease H (RNase H) is an endoribonuclease which specifically degrades the RNA strand of an RNA/DNA hybrid, leaving the DNA strand and unhybridized RNA intact. E.coli RNase H (=RNaseHI) was over-expressed in E. coli as a recombinant protein and highly purified. MW is 17.6 kDa.

Application

• Removal of mRNA in DNA/RNA hybrid prior to the synthesis of the second strand of cDNA (1, 2)
• Removal of poly (A) tails from mRNA after hybridization with oligo (dT) (3)
• Oligodeoxyribonucleotide-directed site-specific cleavage of RNA

References

1) Gubler U (1987) gSecond-strand cDNA synthesis: mRNA fragments as primers.h Method Enzymol 152:
330-335 PMID: 3309563

2) Sambrook J & Russell DW (2001) Molecular Cloning, Chapter 11 gPreparation of cDNA Libraries and Gene
Identificationh. CSHL Press

3) Vournakis JN et al (1975) gElectrophoretic patterns of deadenylylated chorion and globin mRNAs.h

Proc.Natl.Acad.Sci.USA 72: 2959-2963 PMID: 1059086

4) Donis-Keller H (1979) gSite specific enzymatic cleavage of RNA.h Nucleic Acids Res. 7: 179-192 PMID:
386279

Product List

Product Name Cat# Quantity Price

Ribonuclease H DataSheet ,

BAM-02-060-EX

1000 UNIT ¥11,000
$147

Ribonuclease H DataSheet ,

BAM-02-060-5EX

5*1000 UNIT ¥33,000
$440

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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