RecQ DNA helicase, Recombinant


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Background

E. coli RecQ helicase contributes to the genomic stability as the prototype of RecQ family DNA helicases, mutations of which genes are associated with premature aging and cancer susceptibility, known as Bloom’s and Werner’s syndromes (1). RecQ helicase functions in both early and late stages of homologous recombination and unwinds DNAs with particular structures, such as the fork structure for replication, the Holliday junction which is an intermediate of recombination, telomere, quadruplex G4-DNA, etc. In replication fork, it forms a complex with topoisomerase III and regulates DNA topology (1, 2). The product is over-produced as a recombinant protein and highly purified by several steps of chromatography. A single band is observed by SDS-PAGE at 64 kD (Fig 1).

Application

1) Studies on mechanisms of DNA recombination, replication and repair.

2) Application for genetic engineering. For dissociation of special structure DNA.

 

 Fig.1 Polyacrylamide gel electrophoresis of RecQ protein.

Storage:

Store at -70°C

References

1) Hickson I "RecQ helicases: caretakers of the genome.h Nat. Rev. Cancer 3:169-78 (2003) Review PMID: 12612652*
2) Hishida T et al gRole of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks.hGenes Dev 18:1886-1897 (2004) PMID: 15289460
*This product was used in Ref. 2

Product List

Product Name Cat# Quantity Price

RecQ DNA helicase Escherichia coli DataSheet

BAM-01-003-EX

20 UG ¥42,000
$560

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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