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E. coli Single-stranded DNA Binding Protein (SSB)


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Background

E. coli single-stranded DNA binding protein (SSB) binds to single-stranded DNA with high specificity (1, 2). It is involved in DNA replication and recombination in vivo. The SSB gene was expressed as the recombinant protein in E.coli highly purified. The molecular mass is 18.9 kDa.

Application

1) Functional single-stranded DNA-binding protein for studying DNA replication and recombination
2) Enhancement of the specificity and yield of PCR

Form: 5 mg/ml in 20mM Tris-HCl (pH 7.6), 200mM NaCl, 1mM dithiothreitol, 1mM EDTA, 50% glycerol
Quality: Greater than 95% purity as determined by SDS-PAGE (CBB staining) The absence of endonucleases and exonucleases was confirmed.
Data Link: Swiss-Prot P0AGE0
Storage: Store at -20°C
Fig.1 SDS-PAGE of E.coli SSB protein Fig.2 Binding activity to single-stranded DNA
0.02 ug/ul of M13mp18ssDNA was incubated with 0 (lane0), 0.025 (lane1), 0.05 (lane2), and 0.1 (lane3) ug/ul of SSB at 37oC for 30 min. and then 10ul aliquot was subjected to electrophoresis in agarose.
References

1) Krauss,G. et al. (1981) gEscherichia coli single-strand deoxyribonucleic acid binding protein: Stability,
specificity, and kinetics of complexes with oligonucleotides and deoxyribonucleic acid.h Biochemistry 20:
5346-5352 PMID: 7028102
2) Weiner,J.H. et al. (1975) hThe deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and
functions in replication.h J.Biol.Chem. 250:1972-1980 PMID: 1090613

Product List

Product Name Cat# Quantity Price

single-stranded DNA binding protein (SSB) Escherichia coli DataSheet

BAM-02-042-EX

200 UG ¥9,500
$127

single-stranded DNA binding protein (SSB) Escherichia coli DataSheet

BAM-02-042-5EX

5*200 UG ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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