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Taq Single-stranded DNA Binding Protein (SSB)


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Background

Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNA replication and recombination (2). Thermus aquaticus (Taq) SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.

Application

• Stabilizes single-stranded DNA in DNA replication, repair, and recombination

Concentration: 0.5 mg/ml
Form: 50mM Tris-HCl (pH 8.0), 200mM NaCl, 0.1mM dithiothreitol, 0.5mM EDTA, 50% glycerol
Activity: Single-stranded DNA binding activity was confirmed (Fig.2).
Quality Assurance: Greater than 95% of protein determined by SDS-PAGE (CBB staining) The absence of endonucleases and exonucleases was confirmed.
Data Link: UniProtKB/Swiss-Prot Q9KH06 (SSB_THEAQ)
Storage: Store at -20°C
Fig.1 SDS-PAGE of E.coli SSB protein Fig.2 Binding activity to single-stranded DNA
0.02 ug/ul of M13mp18ssDNA was incubated with 0 (lane 0), 0.025 (lane 1), 0.05 (lane 2), and 0.1 (lane 3) ug/ul of SSB at 37?‹C for 30 min and then 10ul aliquot was subjected to electrophoresis in agarose.
References

1) Dabrowski S et al (2002) gNovel thermostable ssDNA-binding proteins from Thermus thermophilus and T.
aquaticus-expression and purification.h Protein Expr Purif. 26: 131-138 PMID: 12356480
2) Greipel J et al (1989) In Saenger,W. and Heinemann,U.(eds), Protein-Nucleic Acid Interaction, Macmillan,
London, pp.61-86

Product List

Product Name Cat# Quantity Price

Taq single-stranded DNA binding protein (SSB) DataSheet ,

BAM-02-044-EX

100 UG ¥20,000
$267

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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