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BINKIT (R) for NK cells expansion from PBMCs


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Feature and Advantages

ENatural killer (NK) cells can be expanded from PBMCs without feeder cells.
EThe number of NK cells will increase several hundred to several thousand-fold in 3 weeks. NK cells will gain strikingly enhanced cytotoxic activity.
EThis kit is suitable for PBMCs from 20 - 50ml of whole blood.
EXeno-free (free from animal-derived materials) EFor research use only

Kit component


Kit nameCatalog No. Amount
BINKIT®   N501-1 1 kit
N501-2 2 kit
N501-4 4 kit
N501-8 8 kit

Contents

ENK Cell Initial Flask (75 cm2):1 flask
ENK Cell Initial Medium:45 ml
ENK Cell Initial Cocktail:1.9 ml
ENK Cell Subculture Medium:1000 ml

Options
ECulture bag(small, large)
ECulture bag connection tube

Others

Example 1, vivo NK cell expansion using BINKIT(R)

NK cells were expanded from peripheral blood mononuclear cells (PBMCs) using BINKIT®. CD3-CD56+ cells were enriched from 15.8% to 88.6%.

Example 2, vivo NK cell expansion using BINKIT(R)

 

NK cells were expanded using BINKIT® from peripheral blood mononuclear cells (PBMCs) of healthy donors (HD; n=3). Cell count (left) and fold changes (right) indicate the efficient ex vivo expansion of NK cells.

Example 3, vivo NK cell expansion using BINKIT(R)

 
NK cell cytotoxicity against K562cell line was measured for BINKIT-expanded NK cells (solid lines) and freshly isolated NK cells (dashed lines) from three independent donors. The expanded NK cells exhibited significantly enhanced cytotoxicity against K562 cell line compared with the freshly isolated NK cells (P < 0.01). ;
References

žXuewen Deng@et al ., Synergistic
cytotoxicity of ex vivo expanded natural killer cells in combination with monoclonal antibody
drugs against cancer cells, Int Immunopharmacol 14 (2012) 593-605

žXuewen Deng et al ., Ex vivo expanded natural killer cells can
possibly kill cancer stem cells, 18th ISCT Annual Meeting 2012

žXuewen Deng et al ., Cytotoxicity of expanded NK cells against
cancer cells is enhanced by monoclonal antibody drugs, 19th ISCT Annual Meeting 2013

Product List

Product Name Cat# Quantity Price

BINKIT(R) for NK cells expansion from PBMCs DataSheet ,

BIJ-N501-1

1 KIT ¥176,000
$2347

BINKIT(R) for NK cells expansion from PBMCs DataSheet ,

BIJ-N501-2

2 KIT contact us

BINKIT(R) for NK cells expansion from PBMCs DataSheet ,

BIJ-N501-4

4 KIT contact us

BINKIT(R) for NK cells expansion from PBMCs DataSheet ,

BIJ-N501-8

8 KIT contact us

NK cells, Human DataSheet

BIJ-FN100-1

1 VIAL ¥30,000
$400

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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