New oxidative stress marker

Anti Nitroguanosine polyclonal antibody


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Background

8-Nitroguanosine is a nitrated nucleic acid which is formed by peroxynitrite, myeloperoxidase, nitrite, and peroxide. It is known that the nitration of guanine is enhanced in virus infection1, 2, bacterial infection3, 4, inflammatory disease5, cancers5, and diseases associated with smoking6. 8-nitroguanosine is thought to be one of the makers of DNA damage caused by oxidative stress. Cyclic GMP (cGMP) is one of the important substances for the signal transfer. On the other hand, 8-Nitro-cGMP (nitrated cGMP) has been identified in vivo3. Therefore, 8-Nitro-cGMP can potentially act as a mediator for reactive oxygen signaling3, 7. Anti-Nitroguanosine polyclonal antibody, does not cross-react with normal nucleobases, it selectively reacts with nitrated nucleic acid such as nitroguanosine, nitroguanine, and nitroxanthine. Therefore, Anti-Nitroguanosine polyclonal antibody is universal antibody of nitrated guanine which modified 8th position of guanine with nitro group. Anti-Nitroguanosine polyclonal antibody has very high affinity for 8-nitroguanine and 8-nitroguanosine, but it does not cross-react with normal guanosine, guanine, 8-hydroxyguanine or 3-nitrotyrosine. Since this antibody was prepared using rabbits, it can be used for immuno-histostaining of rodent tissues.

Application

Immunohistochemistry, 10 ug/ml
ELISA, 10 ug/ml

 

[The Reactivity of Aniti-Nitroguanosine polyclonal antibody]

[Immunostaining example of influenza virus-infected mouse lung epithelial cell]

<References>
1) T. Akaike, S. Okamoto, T. Sawa, J. Yoshitake, F. Tamura, K. Ichimori, K. Miyazaki, K. Sasamoto and H. Maeda, 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis, Proc. Natl. Acad. Sci. USA, 100, 685-690 (2003).
2) J. Yoshitake, T. Akaike, T. Akuta, F. Tamura, T. Ogura, H. Esumi, and H. Maeda, Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity, J. Virol., 78, 8709-8719 (2004).
3) T. Sawa, M. H. Zaki, T. Okamoto, T. Akuta, Y. Tokutomi, S. Kim-Mitsuyama, H. Ihara, A. Kobayashi, M. Yamamoto, S. Fujii, H. Arimoto, and T. Akaike, Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate, Nat. Chem. Biol., 3, 727-735 (2007).
4) M. H.Zaki, S. Fujii, T. Okamoto, S. Islam, S. Khan, K. A. Ahmed, T. Sawa, and T. Akaike, Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis, J. Immunol., 182, 3746-3756 (2009).
5) Y. Terasaki, T. Akuta, M. Terasaki, T. Sawa, T. Mori, T. Okamoto, M. Ozaki, M. Takeya and T. Akaike, Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis, Am. J. Respir. Crit. Care. Med., 174, 665-673 (2006).
6) T. Sawa, M. Tatemichi, T. Akaike, A. Barbin and H. Ohshima, Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking, Free Radic. Biol. Med., 40, 711-720 (2006).
7) K. A. Ahmed, T. Sawa, T. Akaike, Protein cysteine S-guanylation and electrophilic signal transduction by endogeneous nitro-nucleotides, Amino Acids, in press (2010).

Product List

Product Name Cat# Quantity Price

Anti Nitroguanosine (Rabbit) Unlabeled DataSheet

CAC-KMU-P01

200 UG ¥37,000
$494

Product Name Cat# Quantity Price

Anti 8-OHdG (Mouse)  DataSheet

NNS-MOG-020P-EX

20 UG ¥33,300
$444

Anti 8-OHdG (Mouse)  DataSheet

NNS-MOG-100P-EX

100 UG ¥128,000
$1707

Anti Thymidine Glycol (TG) (Mouse) Unlabeled DataSheet

NNS-MTG100P-EX

100 UG ¥76,800
$1024

Anti Nitroguanosine (Rabbit) Unlabeled DataSheet

CAC-KMU-P01

200 UG ¥37,000
$494

Anti Nitroguanosine (Mouse) Unlabeled DataSheet

CAC-KMU-M01

200 UG ¥40,000
$534