New oxidative stress marker

Anti Nitroguanosine monoclonal antibody


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Background

8-Nitroguanosine is a nitrated nucleic acid which is formed by peroxynitrite, myeloperoxidase, nitrite, and peroxide. It is known that the nitration of guanine is enhanced in virus infection1, 2, bacterial infection3, 4, inflammatory disease5, cancers5, and diseases associated with smoking6. 8-nitroguanosine is thought to be one of the makers of DNA damage caused by oxidative stress. Cyclic GMP (cGMP) is one of the important substances for the signal transfer. On the other hand, 8-Nitro-cGMP (nitrated cGMP) has been identified in vivo3. Therefore, 8-Nitro-cGMP can potentially act as a mediator for reactive oxygen signaling3, 7-9. Although the product, Anti-Nitroguanosine monoclonal antibody (NO2G52), does not cross-react with normal nucleotide bases, it selectively reacts with nitro functionalized nucleotides such as nitro-cGMP, nitro-GMP, and nitro-GTP. Therefore, Anti-Nitroguanosine polyclonal antibody is universal antibody of nitrated guanine which modified 8th position of guanine with nitro group. NO2G52 is commonly used for immunostaining. In addition, NO2G52 can potentially utilized for affinity purification of nitroguanine derivative3,6.

Application

Immunohistochemistry, 10 ug/ml
ELISA, 1 ug/ml 

[The reactivity of Monoclonal Antibody(NO2G52)]

 

[Production of 8-nitroguanine by airway epithelial cell with ldiopathic Fibrosis (IPS)]

A. 8-nitroguanine (green) and iNOS (red) 
B. Bright-field 
C. 8-nitroguanine (green) and 8-oxoguanine (Red)
D. 8-nitroguanine (green) and mitochondria (red)

 

[Endogenous guanine nitration in RAW 264.7 cells, a murine macrophage cell line]

<References>
1) T. Akaike, S. Okamoto, T. Sawa, J. Yoshitake, F. Tamura, K. Ichimori, K. Miyazaki, K. Sasamoto and H. Maeda, 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis, Proc. Natl. Acad. Sci. USA, 100, 685-690 (2003).
2)       J. Yoshitake, T. Akaike, T. Akuta, F. Tamura, T. Ogura, H. Esumi, and H. Maeda, Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity, J. Virol., 78, 8709-8719 (2004).
3) T. Sawa, M. H. Zaki, T. Okamoto, T. Akuta, Y. Tokutomi, S. Kim-Mitsuyama, H. Ihara, A. Kobayashi, M. Yamamoto, S. Fujii, H. Arimoto, and T. Akaike, Protein S-guanylation by the biological signal 8-nitroguanosine 3',5'-cyclic monophosphate, Nat. Chem. Biol., 3, 727-735 (2007).
4) M. H.Zaki, S. Fujii, T. Okamoto, S. Islam, S. Khan, K. A. Ahmed, T. Sawa, and T. Akaike, Cytoprotective function of heme oxygenase 1 induced by a nitrated cyclic nucleotide formed during murine salmonellosis, J. Immunol., 182, 3746-3756 (2009).
5) Y. Terasaki, T. Akuta, M. Terasaki, T. Sawa, T. Mori, T. Okamoto, M. Ozaki, M. Takeya and T. Akaike, Guanine nitration in idiopathic pulmonary fibrosis and its implication for carcinogenesis, Am. J. Respir. Crit. Care. Med., 174, 665-673 (2006).
6) T. Sawa, M. Tatemichi, T. Akaike, A. Barbin and H. Ohshima, Analysis of urinary 8-nitroguanine, a marker of nitrative nucleic acid damage, by high-performance liquid chromatography-electrochemical detection coupled with immunoaffinity purification: association with cigarette smoking, Free Radic. Biol. Med., 40, 711-720 (2006).
7) M. Feelisch, Nitrated cyclic GMP as a new cellular signal, Nat. Chem. Biol., 3, 687-688 (2007).
8) K. A. Ahmed, T. Sawa, T. Akaike, Protein cysteine S-guanylation and electrophilic signal transduction by endogeneous nitro-nucleotides, Amino Acids, in press (2010).
T. Sawa, H. Arimoto and T. Akaike, Regulation of redox signaling involving chemical conjugation of protein thiols by nitric oxide and electrophiles, Bioconjugate Chem., in press (2010).

Product List

Product Name Cat# Quantity Price

Anti Nitroguanosine (Mouse) Unlabeled DataSheet

CAC-KMU-M01

200 UG ¥40,000
$534

Product Name Cat# Quantity Price

Anti 8-OHdG (Mouse)  DataSheet

NNS-MOG-020P-EX

20 UG ¥33,300
$444

Anti 8-OHdG (Mouse)  DataSheet

NNS-MOG-100P-EX

100 UG ¥128,000
$1707

Anti Thymidine Glycol (TG) (Mouse) Unlabeled DataSheet

NNS-MTG100P-EX

100 UG ¥76,800
$1024

Anti Nitroguanosine (Rabbit) Unlabeled DataSheet

CAC-KMU-P01

200 UG ¥37,000
$494

Anti Nitroguanosine (Mouse) Unlabeled DataSheet

CAC-KMU-M01

200 UG ¥40,000
$534