Anti Collagen type XII Monoclonal antibody


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Background

Type XII collagen is a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen subfamily curently composed of 9 members (collagen types VII, IX, XII, XIV, XVI, XIX, XX, and XXI). Type XII collagen is a homotrimer composed of three ?1(XII) chains with an approximate Mr of 220 kDa. This collagen was originally found in prevalent association with type I collagen in tendons, ligaments, perichondrium, and periosteum and all dense connective tissues, but more recent studies also document association of the collagen with basement membranes. The type XII-type I collagen connection is thought to modify the interactions between collagen type I fibrils and the surrounding matrix such as to create specific macromolecular configuration of the ECM. Alternatively spliced transcript variants encoding different isoforms of the collagen have been identified. Rotary shadowing/TEM analyses shows a structure with a thin 75-nm tail, which is frequently kinked, attached through a central globule to three finger-like projections, each 60 nm long.

Application

Recommended Antibody Dilutions
    ELISA : 1/10 – 1/250
    Western blotting : 1/10 to 1/50
        ~220 kDa SDS-PAGE under reducing conditions; >600 kDa under non-reducing conditions
     Immunohistochemistry : 1/25 to 1/100 (on frozen tissue sections)
    Immunoprecipitation : 1/5 – 1/10

Product List

Product Name Cat# Quantity Price

Anti Collagen 12 (378D5), Human (Mouse Mono) Unlabeled DataSheet

CAC-PRPG-CO12-M01

2 ML ¥50,000
$667

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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