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useful for the research of various aging disease

Anti D-ƒÀ-Aspartic acid polyclonal antibody


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Background

Biologically uncommon D-aspartyl (Asp) residues have been reported in proteins of the tooth, eye lens, aorta, and brain in elderly humans. Aspartic acid is the most easily racemizable amino acid and D-Asp may be formed by racemization in metabolically inactive tissues during the chronological aging process. We prepared a highly specific polyclonal antibody against peptide Gly.Leu.D-β-Asp.Ala.Thr.Gly.Leu.
-D-b-Asp.Ala.Thr.Gly.Leu.D-β-Asp.Ala.Thr (anti-peptide 3R antibody) which corresponds to three repeats of positions 149.153 of human αA-crystallin. This antibody can distinguish the configuration of the Asp-residue, that is, it reacts very strongly with the D-β-Asp-containing peptide but not react with the L-α-Asp-, L-β-Asp- and D-α-Asp-containing peptides.

<References>
1) Fujii, N., Shimo-oka, T., Ogiso, M., Momose, Y., Kodama, M., & Akaboshi, M., Localization of biologically uncommon D-β-aspartate-containingαA-crystallin in human eye lens, (2000) Mol. Vis., 6, 1-5.
2) Fujii, N., Tajima, S., Tanaka, N., Fujimoto, N., Takata, T., Shimo-Okac, T., The presence of D-b-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging, (2002) BBRC 294, 1047-1051

Application

Recommended use : WB, IHC (Paraffin), ELISA

< Immunostaining >

Immunoreactivity of the antibody for D-b-Asp-containing peptide (peptide 3R) in the skin.

Skin specimens, obtained from the sun-exposed area (face) of 9-year-old (A), 59-year-old (B), and 86-yearold (C), or sun-protected area (buttock) of 85-year-old (D), were embedded in paraffin and then immunoreacted with the antibody for peptide 3R at 1:100 dilution for 24 h
The sections were incubated with rabbit anti-Ig antibody at 1:50 dilution for 2h. Antigen.antibody complex was visualized by avidin.biotin complex (60 in A, B and D, 100 in C).

< References >
1) Fujii, N., Shimo-oka, T., Ogiso, M., Momose, Y., Kodama, M., & Akaboshi, M., Localization of biologically uncommon D-β-aspartate-containingαA-crystallin in human eye lens, (2000) Mol. Vis., 6, 1-5.
2) Fujii, N., Tajima, S., Tanaka, N., Fujimoto, N., Takata, T., Shimo-Okac, T., The presence of D-b-aspartic acid-containing peptides in elastic fibers of sun-damaged skin: a potent marker for ultraviolet-induced skin aging, (2002) BBRC 294, 1047-1051

Product List

Product Name Cat# Quantity Price

Anti D-(β)-Aspartic acid (Rabbit) Unlabeled DataSheet

CAC-KUF-P001

100 UL N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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