Anti Atg16L polyclonal antibody


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Background

Autophagy is an evolutionaly conserved machinery, in which autophagosome fuses with lysosome and degrades bulk cytoplasmic contents. Autophagy is involved in many physiological processes such as development, infection, cancer, and neurodegenerative diseases. ATG (autophagy-related) genes were identified by genetic screening in yeast. Atg7 acts as an E1-like enzyme in both Atg12 and Atg8 ubiquitin-like conjugation systems. Atg7 transfers Atg12 to an E2-like enzyme Atg10, and conjugates Atg12 to Atg5. In the other hand, Atg7 transfers Atg8 to another E2-like enzyme Atg3, and conjugates Atg8 to phosphatidylethanolamine. Many of these ATG genes are conserved also in mammals. Atg7 deficient neonates die soon after birth as they cannot endure perineonatal starvation. Conditional deletion of Atg7 in nerves system results in neurodegeneration with ubiquitin containing aggregates.

Application

Western Blot: 1/1,000-1/2,000.
Predicted molecular weight: 63, 71, 75 kDa (α, β, γ isoforms)
Immunocytochemistry: 1/100-1/200

CAC_TMDPHAT16L_1

Mouse embryonic fibroblasts cultured in the absence of serum and amino acids for 2 hours were subjected to immunocytochemistry using anti-Atg16L antibody (p63C2, 1:200) and a secondary antibody (Alexa fluoro 488-conjugated goat anti-rabbit IgG).

References

1. Mizushima, N., Kuma, A., Kobayashi, Y., Yamamoto, A., Matsubae, M., Takao, T., Natsume, T., Ohsumi, Y. and Yoshimori, T. Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate. J. Cell Sci. 116, 1679-1688. (2003).

Product List

Product Name Cat# Quantity Price

Anti Atg16L, Mouse (Rabbit)  DataSheet

CAC-TMD-PH-AT16L

100 UL N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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