Anti Atg12 polyclonal antibody


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Background

Autophagy is an evolutionaly conserved machinery, in which autophagosome fuses with lysosome and degrades bulk cytoplasmic contents. Autophagy is involved in many physiological processes such as development, infection, cancer, and neurodegenerative diseases. ATG (autophagy-related) genes were identified by genetic screening in yeast. Atg7 acts as an E1-like enzyme in both Atg12 and Atg8 ubiquitin-like conjugation systems. Atg7 transfers Atg12 to an E2-like enzyme Atg10, and conjugates Atg12 to Atg5. In the other hand, Atg7 transfers Atg8 to another E2-like enzyme Atg3, and conjugates Atg8 to phosphatidylethanolamine. Many of these ATG genes are conserved also in mammals. Atg7 deficient neonates die soon after birth as they cannot endure perineonatal starvation. Conditional deletion of Atg7 in nerves system results in neurodegeneration with ubiquitin containing aggregates.

Application

Western Blot: 1/1,000-1/2,000.
Predicted molecular weight: 55 kDa (detected as an Atg12-Atg5 conjugate)
Immunocytochemistry: 1/100-1/200

CAC_TMDPHAT12_1

Total cell lysates were prepared from Atg5+/+ (WT) and Atg5-/- (KO) mouse embryonic fibroblasts and subjected to immunoblotting with anti-Atg12 antibody (1:2000). Note
that almost all Atg12 is conjugated with Atg5 and Atg12 monomer is not detected.

References

1. Mizushima, N., Yamamoto, A., Hatano, M., Kobayashi, Y., Kabeya, Y., Suzuki, K., Tokuhisa, T., Ohsumi, Y. and Yoshimori, T. Dissection of Autophagosome Formation using Apg5-Deficient Mouse Embryonic Stem Cells J. Cell Biol. 152, 657-667. (2001)

Product List

Product Name Cat# Quantity Price

Anti Atg12, Mouse (Rabbit)  DataSheet

CAC-TMD-PH-AT12

100 UL N/A

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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