• HOME
  • Products
  • Counter-Diffusion Protein Crystallization Kit [Crystal Tube Kit]

Counter-Diffusion Protein Crystallization Kit [Crystal Tube Kit]


print

Background

Counter-diffusion is a crystallization method in a capillary in which a protein and a reservoir solution diffuse into each other from opposite directions.1), 2)  Both the protein and the precipitant solutions are set to diffuse through the gel, and the concentration gradients of both solutions form in the capillary. The capillary can continuously scan a wide range of crystallization conditions unless crystallization occurs. By fixing the precipitant concentration higher, you can scan a wider range of crystallization conditions. Therefore, a single capillary may be equivalent to many drops in the vapor-diffusion method.

<Reference>
1) McPherson A., Crystallization of Biological Macromolecules, Cold Spring Harbor Lab. Press (1999)
2) Garcia-Ruiz, J.M., Moreno, A.: Acta Cryst., D50, 484-490(1994)

Feature and Advantages

♦ Size: Very little space is required for crystallization set-up.

♦ Easy Set-up: The Gel-tube already contains agarose in the silicon tube, making the crystallization set up very easy.

♦ Small Protein Sample: Only 2 micro-L per screening. Only 10 micro-L per diffractive-grade crystal.

♦ Favorable Crystallization Conditions: The timing of crystallization can be controlled by Gel-tube length, the  amount of protein solution in a capillary, and the concentration of protein and precipitant solution.

♦ High Reproducibility and Reliability: JAXA uses this method for crystallization in space. After crystallizing over 400 different proteins, the Crystal Tube kit has proven its reliability and high reproducibility. 

♦ Long-term Stability of Crystals: Crystals grown in the capillary are stable over the long term, making it easy to soak crystals with another compound, such as a ligand or cryoprotectant.

♦ Membrane Protein Crystallization: Phase separation due to concentrated detergent in the solution does not occur.

♦ ♦User Manual: Download our user manual at http://www.confsci.co.jp/c-tube.html to learn about crystallization using counter-diffusion method.

Application

< Protein Crystals Grown in the Crystal Tube >

 

< Example >
Buffer + 30% PEG 4000 + 0–700 mM NaCl

Conditions in which crystals, oil and/or precipitate were observed are indicated by “C”, “O” or “P”, respectively.

  • There is an optimum salt concentration for PEG as the precipitant, as shown in the table. If too low, crystals will not grow. If too high, precipitate or oil will be obtained.
  • Crystallization can sometimes be accelerated if the protein and the reservoir solutions are pre-mixed 1:1 before crystallization set-up.
    Reference  ICCBM12 (2008) Program & Abstracts Book 128
Product List

Product Name Cat# Quantity Price

Crystal-Tube GT-R DataSheet ,

CFS-MB2004-CRT200

1 SET ¥90,000
$1200

Product Name Cat# Quantity Price

Screw-top test tube DataSheet ,

CFS-MB2004-CRT201

24 UNIT ¥55,000
$734

Gel-Tubing DataSheet ,

CFS-MB2004-CRT202

1 UNIT ¥24,000
$320

Capillary, 0.3mm i.d DataSheet ,

CFS-MB2004-CRT203

30 UNIT ¥24,000
$320

Capillary, 0.5mm i.d DataSheet ,

CFS-MB2004-CRT204

20 UNIT ¥19,000
$254

Starters kit DataSheet ,

CFS-MB2004-CRT209

1 SET ¥15,000
$200

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


“Counter-Diffusion Protein Crystallization Kit [Crystal Tube Kit]” also belongs to the following categories.