Screen test samples for their ability to inhibit AGEs formation!

Collagen Glycation Assay Kit


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Background

The non-enzymatic reaction of reducing carbohydrates with lysine or arginine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins.

Many AGEs have fluorescent and convalent cross-linking properties. Accumulation of AGEs has been thought to play an important role in the pathogenesis of diabetic patients as well as the aging process.

Recent studies have suggested that AGEs can arise not only from sugars but also from carbonyl compounds derived from the autoxidation of sugars and other metabolic pathways. Among different AGEs, there is evidence that glyceraldehyde -derived AGEs are associated with such cytotoxcity.

"Collagen Glycation Assay Kit, Glyceraldehyde" provides rapid detection of fluorescent AGEs and inhibition assay for glycation of collagen by glyceraldehyde. This kit provides sufficient reagents to perform up to 192 assays.

"Collagen Glycation Assay Kit, Glucose / Fructose" is available for a long term examination that reserch early saccharification from fructose.

The availability and convenience of this Kit will contribute to functional foods or development of anti-glycation material in the cosmetic field.

Feature and Advantages

œ All reagents necessary are included. (plate is not included in a kit)
œ Simple and easy protocol. It can measure in fluorescence plate leaders (excitation wavelength 370nm, fluorescence wavelength 440nm).
œ It can assay two pieces of 96well plates.
œ It is suitable for searching material that controlls collagen saccharification.


AK70 is available for the examination that reserch early saccharification from fructose.It can examine saccharification from fructose for a long term with time.

AK71 is to use glyceraldehyde which is a glucose metabolism intermediate, and a result is provided after a 24-hour short reaction.

Kit component

 

yAK 70-COSz

AK70-COS Kit Component
ComponentsQuantityStorage
Collagen Acidic Solution 5 mL     4c°
Neutralizing Solution 5 mL
Glucose solution (200 mM) 10 mL
Fructose solution (200 mM) 10 mL
Sample Dilution Buffer 20 mL
Aminoguanidine Solutioni20mMj: Positive control 0.1 mL

 

yAK 71-COSz

AK71-COS Kit Component
Components Quantity Storage
Collagen Acidic Solution 5 mL     4c°
Neutralizing Solution 5 mL
Glyceraldehyde Solutioni500mMj 2 mL
Sample Dilution Buffer 30 mL
Aminoguanidine Solutioni20mMj: Positive control 0.5 mL

 

Difference between AK70-COS and AK71-COS

Cat# Product name Glycation starter materials Time necessary for an assay
PMC-AK70-COS Collagen Glycation assay kit, Glucose / fructose Glucose / fructose 10-30 days
PMC-AK71-COS Collagen Glycation Assay Kit, Glyceraldehyde Glyceric aldehyde 1 day

Experimental Result

Glycation of Collagen gel-Glyceraldehyde
This figure demonstrates Collagen Glycation Assay Kit, Glyceraldehyde results that inhibitory effects of aminoguanidin, Houttuynia cordata extraction, and Asparagus extraction on collagen glycation.
Product List

ico_caution_s.pngThis kit  can assay two pieces of 96well plates. 

 


Product Name Cat# Quantity Price

Collagen Glycation Assay Kit, Glucose / Fructose DataSheet ,

PMC-AK70-COS

1 KIT ¥36,000
$480

Collagen Glycation Assay Kit, Glyceraldehyde DataSheet ,

PMC-AK71-COS

1 KIT ¥54,000
$720

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


“Collagen Glycation Assay Kit” also belongs to the following categories.