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This immunoassay kit allows for the in vitro quantitative determination of mouse PIIINT
concentrations in cell culture supernates, serum, plasma and other biological fluids..

Mouse procollagen III N-terminal peptide (PIIINT) ELISA Kit


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Background

Procollagen type III is synthesized in fibroblasts as a biosynthetic precursor of collagen type III and then released. The propeptides are split off in the extracellular space during the conversion into collagen. Type III procollagen N-terminal peptide, a peptide released during the conversion of type III procollagen to type III collagen is considered a potential marker of fibroblast activity in a variety of pulmonary and extrapulmonary diseases.

Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with an antibody specific to P? NT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for P?NT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain P?NT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of P?NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

< SPECIFICITY>
This assay recognizes recombinant and natural mouse P?NT. No significant cross-reactivity or interference was observed.

<DETECTION RANGE>
0.312 ng/ml-20 ng/ml. The standard curve concentrations used for the ELISA’s were 20 ng/ml,10 ng/ml, 5 ng/ml, 2.5 ng/ml, 1.25 ng/ml, 0.625 ng/ml, 0.312 ng/ml.

<SENSITIVITY>
he minimum detectable dose of mouse P?NT is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.

Kit component

   Reagent Quantity
1. Assay plate 1
2. Standard 2
3. Sample Diluent 1 x 20 ml
4. Biotin-antibody Diluent 1 x 10 ml
5. HRP-avidin Diluent 1 x 10 ml
6. Biotin-antibodyt 1 x 120μl
7. HRP-avidin 1 x 120μl
8. Wash Buffer 1 x 20 ml (25×concentrate)
9. TMB Substrate 1 x 10 ml
10. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

CSB-E07928M
Assay range : 0.312 ng/ml- 20 ng/ml
Assay sample volume : 100 µL (Cell Culture Supernates, Plasma,Serum)
Assay time : 6-8 hr. {3 hr

CSB-E07927R
Assay range : -
Assay sample volume : -
Assay time : -

CSB-E07926H
Assay range : 0.78 ng/ml- 50 ng/ml.
Assay sample volume : 100 µL (Cell Culture Supernates, Plasma,Serum)
Assay time : 6-8 hr. {3 hr


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