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This immunoassay kit allows for the in vitro semi-quantitative determination of mouse GAD(IgG) concentrations in serum, plasma and other biological fluids.

Mouse glutamic acid decarboxylase autoantibody IgG (GAD-IgG) ELISA Kit


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Feature and Advantages

<PRINCIPLE OF THE ASSAY>
The microtiter plate provided in this kit has been pre-coated with purified GAD antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-mouse IgG to each well and incubate. Finally, a TMB (3,3',5,5'tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Calculate the valence of GAD (IgG) in the samples.

< SPECIFICITY>
This assay recognizes mouse GAD (IgG). No significant cross-reactivity or interference was observed.

<SENSITIVITY>
The minimum detectable dose of mouse GAD (IgG) is typically less than 1 ng/ml.

Kit component

  Reagent Quantity
1. Assay plate 1
2. Sample Diluent 1 x 20 ml
3. HRP-anti-mouse IgG Diluent 1 x 10 ml
4. HRP-anti-mouse IgG 1 x 120μl (1:100)
5. Wash Buffer 1 x 20 ml (25×concentrate)
6. TMB Substrate 1 x 10 ml
7. Stop Solution 1 x 10 ml
Product List

Product Name Cat# Quantity Price

CSB-E08655M
Assay range : less than 1 ng/ml
Assay sample volume : 100 µL (Plasma,Serum)
Assay time : 5-6 hr. {3 hr

CSB-E08654R
Assay range : less than 1 ng/ml
Assay sample volume : 100 µL (Plasma,Serum)
Assay time : 5-6 hr. {3 hr

CSB-E06912H
Assay range : less than 1 ng/ml
Assay sample volume : 100 µL (Plasma,Serum)
Assay time : 5-6 hr. {3 hr


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