High sensitivity bioluminescence kit

Luciferase FM PLUS


Luciferase FM is a genetically modified variant of native North American firefly (photinus pyralis) luciferase generating a luminescence intensity approximately 10 times greater than the wild type enzyme (see reverse) and thus, ideally suited for high sensitivity ATP detection. The Luciferase FM Plus Reagent Set includes ATP-free water and an optimally formulated lyophilized premix containing Luciferase FM enzyme, luciferin, and buffer salts to deliver the ultimate sensitivity in ATP detection.

Kit contents : 2 x 5 ml Luciferin FM reagent premix, lyophilized. 30 ml AT-free sterile water, ready to use.

Luciferase FM Plus flyer download [PDF]

Principle of Luciferase FM ATP detection

Principle of Luciferase FM ATP detection

Luciferase FM vs. Luciferase FM Plus

Bioluminescence detection of bacteria with Luciferase FM Plus

1) Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A., Increase in bioluminescence intensity of firefly luciferase using genetic modification. Anal Biochem. 2007 Jul 15; 366 (2): 131-6. PMID:17540326
2) Noda K, Matsuno T, Fujii H, Kogure T, Urata M, Asami Y, Kuroda A., Single bacterial cell detection using a mutant luciferase.Biotechnol Lett. 2008 Jun;30(6):1051-4. PMID:18224280
3) Noda K, Goto H, Murakami Y, Ahmed AB, Kuroda A., Endotoxin assay by bioluminescence using mutant firefly luciferase. Anal Biochem. 2010 Feb 15;397(2):152-5. PMID:19850001

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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