recombinant protein

Cdk5/p25, active


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Background

CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. This recombinant product is a complex of human Cdk5 and C-terminal His-tagged C-terminal p25 fragment of human p35, and was expressed in Sf9 insect cells by baculovirus.

Formulation

10mM MOPS (pH6.8), 0.1M NaCl, 1mM MgCl2, 0.5% Nonidet P-40, 1mM DTT, 10% glycerol, 0.4mM Pefabloc, 1 μM E64.

Specific Activity

500 U/mg, where one unit of cdk5/p25 activity is defined as 1 nmol phosphate incorporated into 0.1 mg/ml Histone H1 per minute at 35 degrees with a final ATP concentration of 100 μM.

CSR_SDT02CP25_01.jpg

< Reference>
1) Saito, T., Onuki, R., Fujita, Y., Kusakawa, G., Ishiguro, K., James, A.B., kishimoto, T. and Hisanaga, S. Developmental Regulation of the Proteolysis of the p35 Cyclin-Dependent Kinase 5 Activator by Phosphorylation. J. Neurosci. 23(4): 1189-1197 (2003)
2) Sakaue, F., Saito, T., Sato, Y., Asada, A., Ishiguro, K., Hasegawa, M. and Hisanaga S. Phosphorylation of FTDP-17 Mutant tau by Cyclin-dependent Kinase 5 Complexed with p35, p25, or p39. J. Biol. Chem. 280(36): 31522-31529 (2005)

Product List

Product Name Cat# Quantity Price

Cdk5/p25 (active), Human DataSheet

CSR-SDT-02-CP25

10 UG ¥30,000
$400