For research of skeletal metabolism

Bone Resorption Assay Kit


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Background

This product is an assay kit to measure bone resorption activity with a fluoresceinated calcium phosphate-coated plate (patent pending).  The coated calcium phosphate is first bound to fluoresceinamine-labeled chondroitin sulfate (FACS), which is released from the calcium phosphate layer into conditioned medium by osteoclastic resorption activity.  Bone resorption activity is evaluated by measuring the fluorescence intensity of the conditioned medium.  Unlike traditional pit assays, rapid evaluation is possible with this system .

BONE RESORPTION ASSAY KIT 24
BONE RESORPTION ASSAY KIT 24

BONE RESORPTION ASSAY KIT 48

 


BONE RESORPTION ASSAY KIT Flyer Download [PDF]

Principle

Features

1) Bone resorption activity is evaluated simply by measuring the fluorescence intensity of the medium.
2) The fluorescence excitation and emission wavelengths are identical to those for the commonly-used fluorescent dye, FITC.
3) Microscopic observation of cell morphology is possible.
4) Pit area analysis is also possible after removing the cells.

Ready to use, sterile components.

Application

[Differentiation into osteoclast by RANKL]


Osteoclastic differentiation of RAW264 cells induced by RANKL (100 ng/mL) was evaluated by measuring the fluorescence intensity of the conditioned medium.  Photographs show pit formation on the calcium phosphate (CaP) coating with or without RANKL stimulation.iœ: with RANKL, ›: without RANKL, *: p<0.05, **: p<0.001)

 

[Comparison between fluorescence intensity and pit area]


RANKL-dependent increase of fluorescence intensity of the conditioned medium (left) and pit area (right) from RAW264 cells in fluoresceinamine-labeled chondroitin sulfate (FACS)/ calcium phosphate (CaP)-coated plates (mean ± SD, n = 3, **: p<0.001).

 

[Drug evaluation]


The inhibitory effects of Pamidronate and β-Estradiol on calcium phosphate resorption induced by RANKL (100 ng/mL) were measured by fluorescence intensity (left) and pit area (right) (mean ± SD, n = 3, *:p<0.05, **: p<0.001).

 

 Method of Measuring Pit Area 

 

References

> Chih-Hsin Kuo et al., arcinogenesis, 34. 11 2600-2609 (2013)
> Claire Fabre, Naoya Mimura, Katharyn Bobb, et al., Clin Cancer Res. 18, 4669-4681 (2012)
> Kim J. et al., Cell. Biochem. 113:247-259 (2012)
# TatsuyaMiyazaki. et al., Analytical Biochemistry 410 7-12 (2011)
# Tatsuya Miyazaki. et al., Dental Materials Journal 29 4:403-410 (2010)

Product List

Product Name Cat# Quantity Price

BONE RESORPTION ASSAY KIT 24 DataSheet ,

CSR-BRA-24KIT

1 KIT ¥66,000
$880

BONE RESORPTION ASSAY KIT 48 DataSheet ,

CSR-BRA-48KIT

1 KIT ¥73,000
$974

BONE RESORPTION ASSAY KIT 48x2 DataSheet ,

CSR-BRA-48X2KIT

2*1 KIT ¥121,000
$1614

Product Name Cat# Quantity Price

BONE RESORPTION ASSAY PLATE 24 DataSheet ,

CSR-BRA-24P

1 PLATE ¥30,000
$400

BONE RESORPTION ASSAY PLATE 48 DataSheet ,

CSR-BRA-48P

1 PLATE ¥37,000
$494

BONE RESORPTION ASSAY PLATE 48x2 DataSheet ,

CSR-BRA-48X2P

2*1 PLATE ¥61,000
$814

BONE RESORPTION ASSAY FACS DataSheet ,

CSR-BRA-FACS1

13 ML ¥37,000
$494

BONE RESORPTION ASSAY BUFFER DataSheet ,

CSR-BRA-B1

10 ML ¥4,000
$54

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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