Useful for the research of skeletal metabolism

Bone Resorption Assay Plate


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Background

This product is a calcium phosphate (CaP)-coated 24-well plate used to measure the bone resorption activity of osteoclasts. The plate is coated with a synthetic CaP (carbonate apatite), similar to that of natural apatite, which is able to be used as an alternative to dentin discs.


BONE RESORPTION ASSAY PLATE 24

BONE RESORPTION ASSAY PLATE 48

 


Bone Resorption Assay Plate Flyer Download  [PDF]

Feature and Advantages

1) Observation of cell morphology is possible using a microscope.
2) Analysis of pit area is also possible using image analyzing software after removing cells.
3) Plate is easy to handle because it is pre-coated with CaP.
4) Plate is sterilized and ready to use

Application

A) Phase-contrast micrograph of RAW264 cells (day 6) cultured in CaP-coated plates stimulated with RANKL (Oriental Yeast Co., Ltd.,Tokyo, Japan; 100 ng/mL). Osteoclast-like cells (OC) were observed.

B) Photograph of the plate after removing cells. Pits can be observed macroscopically (Left: without RANKL; Right: with RANKL).

C) Micrograph of the pits in a CaP-coated plate (with RANKL).

D) RANKL-dependent increase of the pit area (mean ± S.D., n = 3).

E) The inhibitory effect of the bisphosphonate, Pamidronate, on CaP resorption induced by RANKL (100 ng/mL).

Frequently Asked Questions

< Reference >
ETatsuya Miyazaki. et al. Analytical Biochemistry 410 7-12 (2011)
EJung-Lye Kim et al. Journal of Cellular Biochemistry 113 247-259 (2012)
ETatsuya Miyazaki. et al, Dental Materials Journal 29 4:403–410 (2010)

Product List

Product Name Cat# Quantity Price

BONE RESORPTION ASSAY PLATE 24 DataSheet ,

CSR-BRA-24P

1 PLATE ¥30,000
$400

BONE RESORPTION ASSAY PLATE 48 DataSheet ,

CSR-BRA-48P

1 PLATE ¥37,000
$494

BONE RESORPTION ASSAY PLATE 48x2 DataSheet ,

CSR-BRA-48X2P

2*1 PLATE ¥61,000
$814

Product Name Cat# Quantity Price

BONE RESORPTION ASSAY KIT 24 DataSheet ,

CSR-BRA-24KIT

1 KIT ¥66,000
$880

BONE RESORPTION ASSAY KIT 48 DataSheet ,

CSR-BRA-48KIT

1 KIT ¥73,000
$974

BONE RESORPTION ASSAY FACS DataSheet ,

CSR-BRA-FACS1

13 ML ¥37,000
$494

BONE RESORPTION ASSAY BUFFER DataSheet ,

CSR-BRA-B1

10 ML ¥4,000
$54

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