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Food Allergen Screening Test Kit

FASTKIT ELISA Ver. III "PEANUT", "EGG", "WHEAT", "BUCK WHEAT", "MILK", "SESAME" and "SOYBEAN"


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FAST KIT ELISA Ver. III [PDF]

FAST KIT ELISA Ver.III@"SESAME" is comming soon.

Background

This product is a food allergen screening test kit which can detect allergen proteins in foods.This product is based on the guidelines shown by CAA Notification No. 286 from the Deputy Secretary General of the Consumer Affairs Agency dated September 10, 2010, "Test methods for food products containing allergic substances". This product can measure specific proteins in the food such as raw materials and processed foods widely.
According to CAA Notification No. 36 from the Deputy Secretary General of the Consumer Affairs Agency dated March 26, 2014, " Partial Revision of "Test methods for food products containing allergic substances"", This is the improved kit by removing 2-mercaptoethanol from "FASTKIT ELISA Ver.II". In addition, according to a separate attachment 6 of CAA Notification No.36 from the Deputy Secretary General of the Consumer Affairs Agency "Guidelines for Evaluating Improved Methods of the Test Methods for Food Products Containing Allergic Substances",it has been confirmed that its performance is equal to that of "FASTKIT ELISA Ver.II".

Product Features

œThis kit is intended to measure allergen proteins in food products.
œThis kit can measure standard allergen proteins in a solution at a concentration ranging from 0.78 to 50 ng/mL.

Component

A. Antibody-immobilized microtiter plate (with a cover) 96-wells (8 wells x 12 strips) x 1
B. Standard solution (50 ng/mL) 1.8 mL x 1
C. Dilution buffer 100 mL x 1
D. Biotin-conjugated antibody 150 µL x 1
E. Enzyme (peroxidase)-labeled streptavidin 150 µL x 1
F. Chromogenic substrate 12 mL x 1
G. Stop solution (0.5N H2SO4) 12 mL x 1
H. Concentrated washing solution (1/10 concentration) 100 mL x 1
I. Extraction reagent (1) (1/20 concentration) 50 mL x 1
J. Extraction reagent (2) (1/20 concentration) 50 mL x 1
K. Extraction reagent (3) (1/20 concentration) 50 mL x 1
L. Instruction manual 1

Screening Principle

(1) An antibody to bind to more than one allergen protein has been immobilized in the plate wells.

(2) The immobilized antibody captures multiple peanut proteins (p1,p2…) in the measurement solution to form complexes.

(3) The Biotin-labeled antibody binds to the complexes to form sandwich complexes. The Enzyme-labeled streptavidin then binds to the sandwich complexes through the reaction between biotin and streptavidin.
The enzymatic substrate is then added to produce a color.

Product List

Product Name Cat# Quantity Price

FASTKIT ELISA Ver. III BUCKWHEAT DataSheet ,

NPH-999100433EX

1 KIT ¥78,000
$1040

FASTKIT ELISA Ver. III EGG DataSheet ,

NPH-999100430EX

1 KIT ¥78,000
$1040

FASTKIT ELISA Ver. III WHEAT DataSheet ,

NPH-999100431EX

1 KIT ¥78,000
$1040

FASTKIT ELISA Ver. III PEANUT DataSheet ,

NPH-999100432EX

1 KIT ¥78,000
$1040

FASTKIT ELISA Ver. III MILK DataSheet ,

NPH-999100434EX

1 KIT ¥78,000
$1040

FAST ELISA Ver. III Soybean DataSheet ,

NPH-999100435EX

1 KIT ¥78,000
$1040

FAST KIT ELISA Ver.III@"SESAME" is comming soon.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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