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Reagents to stabilise and improve spermatozoa cryopreservation results and in vitro fertilization (IVF) rates for laboratory mice

FERTIUP® Mouse Sperm Cryoprotectant
FERTIUP® Mouse Sperm Preincubation Medium
CARD MEDIUM® Mouse Fertilization Medium
FERTIUP® PM -CARD MEDIUM® set


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Background

Sperm cryopreservation is a practical and cost-effective method of archiving mouse strains in the laboratory. FERTIUP® and CARD MEDIUM® are high-performance media for mouse sperm cryopreservation, sperm preincubation and in vitro fertilization (IVF). When used in combination, FERTIUP® and CARD MEDIUM® provide a high and stable fertilization rate using frozen-thawed mouse sperm. This will allow you to reproduce mice whenever you need them.

The FERTIUP® sperm cryoprotectant and sperm preincubation medium greatly improve fertilization rates of frozen-thawed sperm in C57BL/6 and 129 Mice strains, from less than 10% (using conventional methods) to more than 30%. In addition, our new CARD MEDIUM® enhances the fertility rate to over 80%.

It goes without saying that FERTIUP® and CARD MEDIUM® have no reproduction toxicity of any kind.

Our newly developed system for sperm cryopreservation and IVF using FERTIUP® and CARD MEDIUM® provides superior support for the safe and reliable preservation of your valuable mouse strains.


Fig 1 Offspring derived via in vitro fertilization using cryopreserved sperm treated with FERTIUP® and CARD MEDIUM®.

FERTIUP® and CARD MEDIUM® flyer download [PDF]

What is FERTIUP® & CARD MEDIUM®?

FERTIUP®and CARD MEDIUM® are reagents to stabilise and improve spermatozoa cryopreservation results and in vitro fertilization rates (IVF) for laboratory mice. The series of products consits of the following:


1. FERTIUP® Mouse Sperm Cryoprotectant (KYD-001-EX)
Reagent used when freezing sperm for storage.

2. FERTIUP® Mouse Sperm Preincubation Medium (KYD-002-EX)
→Reagent used to pre-incubate sperm after thawing from frozen state. To perform before IVF.

3.CARD MEDIUM® Mouse Fertilization Medium (KYD-003-EX)
Medium used for IVF

Usage of FERTIUP alone will lead to higher fertilization rates, but combined usage with CARD MEDIUM will lead to even higher fertilization rates and success.

Feature and Advantages

 High fertilization rate (over 80%)
 Improvements in the management of genetically modified mice (Tg/KO)
 Reduction in labor, facilities, and breeding cost.
 Reduction in time required to expand a mouse colony
 Efficient production of strains which are notoriously difficult to breed


Fig. 2 Fertilization rates using frozen-thawed sperm with FERTIUP® and with FERTIUP® and CARD MEDIUM®

 


Fig.3 Fertilizaion rates usign FERTIUP® Preincubation Medium and CARD MEDIUM® for fresh and frozen sperm in various strains.

Manual and Frequently Asked Questions (FAQ)

 Cryopreservation of Mouse Spermatozoa
(Using FERTIUP® Cryoprotectant, HTF, Sperm Straws, Straw connector and Freezing canister)
 In Vitro Fertilization using Cryopreserved Spermatozoa
(Using FERTIUP® Preincubation medium, CARD MEDIUM®, HTF and Straw connector)
 Collection and Transport at Cold Temperature of Cauda Epididymis(Using Card Cold Transport Kit)
 In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
(Using FERTIUP® Preincubation Medium: PM and HTF)
  Transport of 2-Cell Embryos at Cold Temperature
(Using Card Cold Transport Kit, M2 and KSOM)
 In vitro fertilization 
(Using FERTIUP® Preincubation medium, CARD MEDIUM® and HTF)
 Simple Vitrification of Mouse Embryos
(Using DAP213 and DMSO)
 Embryo Transfer into the Oviduct
 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
(Using FERTIUP® Preincubation medium: PM, CARD MEDIUM® and HTF)
 Procedure for Warming of Vitrified Embryos
(Using 0.25M sucrose and KSOM)
 Storage of Media and Solutions in Ampoules Under Nitrogen Gas
 Simple Vitrification of Mouse Oocytes
(Using HTF)
 Tables of Media Composition

1M DMSO, DAP213, 0.25M Sucrose, mHTF and KSOM

 Request for a Hard Copy of the Manual!

Frequently Asked Questions for FERTIUP® & CARD MEDIUM®

A list of FAQ can be found here

   back to "Mouse Reproductive Engineering" page
References

> Takeo, T., Hoshii, T., Kondo, Y., Toyodome, H., Arima, H., Yamamura, KI., Irie, T. and Nakagata, N. Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Biol Reprod. 78(3):546-51. 2008.

> Takeo, T and Nakagata, N. Combination medium of cryoprotective agents containing L-glutamine and methyl-{beta}-cyclodextrin in a preincubation medium yields a high fertilization rate for cryopreserved C57BL/6J mouse sperm. Lab Anim. 44(2):132-7. 2010

> Nakagata N. 2011. Cryopreservation of mouse spermatozoa and in vitro fertilization. Methods Mol Biol. 693: 57-73

> Takeo T., and Nakagata N et al. 2009. Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature. Cryobiology. 58(2): 196-202

> Takeo T., and Nakagata N et al. 2010. Short-term storage and transport at cold temperatures of 2-cell mouse embryos produced by cryopreserved sperm. J Am Assoc Lab Anim Sci. 49(4): 415-419

Product List

FERTIUP®


We offer a variety of sizes available for your convenient usage (0.5ml, 1ml, 5*0.5ml, 5*1ml).
Please note once the ampule is opened, we recommend you to use it up, and not store an opened ampule for future usage.


Product Name Cat# Quantity Price

FERTIUP(R) Cryoprotectant: CPA DataSheet ,

KYD-001-EX

1 ML ¥6,500
$87

FERTIUP(R) Cryoprotectant: CPA DataSheet ,

KYD-001-EX-X5

5*1 ML ¥30,000
$400

FERTIUP(R) Cryoprotectant: CPA DataSheet ,

KYD-001-05-EX

0.5 ML ¥3,800
$51

FERTIUP(R) Cryoprotectant: CPA DataSheet ,

KYD-001-05-EX-X5

5*0.5 ML ¥17,500
$234

Product Name Cat# Quantity Price

FERTIUP(R) Preincubation Medium: PM DataSheet ,

KYD-002-EX

1 ML ¥6,500
$87

FERTIUP(R) Preincubation Medium: PM DataSheet ,

KYD-002-EX-X5

5*1 ML ¥30,000
$400

FERTIUP(R) Preincubation Medium: PM DataSheet ,

KYD-002-05-EX

0.5 ML ¥3,800
$51

FERTIUP(R) Preincubation Medium: PM DataSheet ,

KYD-002-05-EX-X5

5*0.5 ML ¥17,500
$234

CARD MEDIUM®


[Kit Components]
An ampoule including medium (A)
A vial including powder (B)
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22ƒÊm)

Product Name Cat# Quantity Price

CARD MEDIUM(R) DataSheet ,

KYD-003-EX

1 KIT ¥12,000
$160

Set Products (FERTIUP® + CARD MEDIUM)

 

FERTIUP(R) PM 1ML-CARD MEDIUM(R) set (KYD-004-EX)

[Kit Components]
An ampoule including medium (A)
A vial including powder (B)
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22μm)
An ampule of PM (1.0ml) 

FERTIUP(R) PM 0.5ML-CARD MEDIUM(R) set (KYD-005-EX)

[Kit Components]
An ampoule including medium (A)
A vial including powder (B)
A 1.5mL plastic tube (C)
A 1.5mL plastic tube (D)
A 2.5mL disposable syringe
A needle
A filter unit (pore size: 0.22μm) 
An ampule of PM (0.5ml) 


Product Name Cat# Quantity Price

FERTIUP(R) PM 1ML-CARD MEDIUM(R) set DataSheet ,

KYD-004-EX

1 SET ¥16,000
$214

FERTIUP(R) PM 0.5ML-CARD MEDIUM(R) set DataSheet ,

KYD-005-EX

1 SET ¥14,000
$187

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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