Mebiol Gel Frequently Asked Questions


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Questions regarding Physical Properties of Mebiol Gel

Question When the temperature is above 37°C(or even higher up to 60°C) , what happens to the gel?
Answer The gel becomes harder as temperature elevates
Question In what state is the Mebiol Gel at temperatures 2°C to 37°C? What is the exact temperature when the gel changes to solution state?
Answer Sol-gel transition temperature is ca.20°C
Question How is the Carbon Dioxide gas and nutrients supplied to cells? Can they dissolve into the cells at hydrogel state?
Answer Nutrition and CO2 enter cells by diffusion in the gel from a multilayer nutrient medium.
Question When Mebiol Gel is in the gel state, cells will be surrounded by hydrogel. Is there enough space for cell growth?
Answer Mebiol Gel's cross-linked points are reversible even in it's gel state (i.e 37°C). The surrounding gel can change shape in accordance with the cell growth without any gap/spaces.
Question Is there any damage to cells when they are harvested (in solution state)?                     
Answer No
Question Do cells experience any resistance when cell growing in contact with the edge of the hydrogel?
Answer No
Question Can you use this gel to culture human lung epithelial cells? Do you have data for this?
Answer No, unfortunately we do not have any data available.
Question How long does it take for Mebiol Gel® to become a gel form from solution when in appropriate temperature?
Answer 3 msec
Question What is the form of Mebiol Gel®  when the temperature is between 15-25C?
Answer Around the transition temperature, there are sol state and gel state at the same time (intermediate state).
Question I would like to know the porosity of the gel at cell culture conditions (37C). 
Answer

Unfortunately, there isn't any data for gel porosity. However, we have information about "gel diffusive speed in different materials" in the Discussion section of the following paper.

Takao et al., Novel drug delivery system using thermoreversible gelation polymer for malignant glioma Journal of Neuro-Oncology (2005)

Generally, when the gel concentration is low, gel "diffusive speed" in materials is high. It means when the gel concentration is low, gel porosity is thought to be high. However, it doesn't become gel when the gel concentration is low like 5%.

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Questions regarding Mebiol Gel and compatible cell types

 

Question When the temperature is above 37°C(or even higher up to 60°C) , what happens to the gel?
Answer The gel becomes harder as temperature elevates
Question In what state is the Mebiol Gel at temperatures 2°C to 37°C? What is the exact temperature when the gel changes to solution state?
Answer Sol-gel transition temperature is ca.20°C
Question How is the Carbon Dioxide gas and nutrients supplied to cells? Can they dissolve into the cells at hydrogel state?
Answer Nutrition and CO2 enter cells by diffusion in the gel from a multilayer nutrient medium.
Question Can we exchange the medium when cell growing at 37°C?
Answer The gel doesn't dissolve in excessive water at 37°C. Thus, you can layer medium on the gel. And you can exchange this layered medium.
Question When Mebiol Gel is in the gel state, cells will be surrounded by hydrogel. Is there enough space for cell growth?
Answer Mebiol Gel's cross-linked points are reversible even in it's gel state (i.e 37°C). The surrounding gel can change shape in accordance with the cell growth without any gap/spaces.
Question

Is the gel suitable for culturing of  T cells or MSC Cells?

Answer

We do not have any data or experience culturing T cells in mebiol gel. Regarding MSC cells, please refer to the following paper 

Yuguo Lei et al., PNAS PLUS E5039–E5048 doi: 10.1073/pnas.1309408110

Question Is there any damage to cells when they are harvested (in solution state)?                     
Answer No
Question Do cells experience any resistance when cell growing in contact with the edge of the hydrogel?
Answer No
Question Which kind of cells have been successfuly cultured with Mebiol Gel®?
Answer Please refer to our references and citation list to see published papers using Mebiol Gel to culture cells.
Question Has Mebiol Gel® been used to culture intestinal cells? Is there any data available?
Answer No, unfortunately we do not have any data available.
Question Can Mebiol Gel® be used for FACS (Flow Cytometry)? Which state does the Gel have to be in?
Answer Yes, it can be used for FACS in its Sol state (low temperature).
Question Can Mebiol Gel® be used in vivo? How long can it remain inside a mouse?
Answer

Yes, it can be used in vivo (not for human). Mebiol remained in rat brain for more than 28 days as a gel form. You can refer to the following paper for further information.

T. Ozeki, K. Hashizawa, D. Kaneko, Y. Imai, H. Okada, “Treatment of rat brain tumors using sustained-release of camptothecin from poly(lactic-co-glycolic acid) microspheres in a thermoreversible hydrogel”, Chem. Pharm. Bull. 58 (9), 1142-1147 (2010)

Question Can you use this gel to culture human lung epithelial cells? Do you have data for this?
Answer No, unfortunately we do not have any data available.
Question Can Mebiol Gel®  be toxic to certain types of cells? If so, which types of cells would that be?
Answer Mebiol Gel is non-toxic to cells.
Question Is there any data for Mebiol Gel® with Thyroid cells (normal or cancer)?
Answer Yes, there is. Please refer to the following: Production of hepatitis C viruses (HCV) by 3-D culture of human hepatocyte cell lineE “Production of infectious hepatitis C virus particles in three-dimensional cultures of the cell line carrying the genome-length dicistronic viral RNA of genotype 1b“ Virology, 351 (2), 381-392 (2006)
Question Can you use this gel to culture human lung epithelial cells? Do you have data for this?
Answer Unfortunately, we don't have the data for culture of human lung epithelial cells. 

 

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Other Questions

 

Question Do cells experience any resistance when cell growing in contact with the edge of the hydrogel?
Answer No
Question Is there any damage to cells when they are harvested (in solution state)?                     
Answer No
Question How long is the shelf life of Mebiol Gel®? 
Answer Roughly 2 years after the date it has been manufactured.  We will try to ship to you Gels with long expiry dates, but please note they can be short sometimes.
Question I have some remaining gel after using a portion of it. How long can the remaining gel be stored at refrigeration?
Answer Roughly 1 month. For longer desired storage, freeze at -20°C or -80°C.
Question I am interested in trying out Mebiol Gel for my first time. Could I have a sample for Mebiol Gel®?
Answer We are sorry, we do not provide samples for Mebiol Gel. 
Question How is Mebiol Gel® sterilized? Is there a possibility that there are some living organisms in the gel?
Answer By EOG sterilization. There should not be any living organisms in the gel.
Question Can Mebiol Gel® be used for FACS (Flow Cytometry)?
Answer Yes, it can be used for FACS in its Sol state (low temperature).
Question What size flask is used for the 10ml and 50ml Mebiol Gel® product?
Answer

T-25 and T-75 Flask is used, respectively.

Question Can I reconstitute it at 20% instead of 10% for stock storage? I would like to be able to dilute it later with a different type of medium.
Answer
There is no problem that your customer reconstitute it at 20% instead of 10% for stock storage.
However,please note that 20% high-concentration reconstitution has high degree of viscosity and has low flowability even though under low temperature.
There is no problem to reconstitute it at 20% instead of 10% for stock storage. However, please note that 20% high-concentration reconstitution has a high degree of viscosity and has low flowability even under low temperature.
Question Mebiol Gel® (MBG-PMW20-1001) is said to be dissolved in 10ml culture media. Can it be dissolved in bigger volume of culture media?
Answer We usually recommend 10ml of Mebiol to be dissolved in 10ml of culture medium. (when you would like to dissolve 50ml of MebiolGel, you should add 50ml of culture medium) If you make low concentration of MebiooGel solution, the solution cannot be gel state. For example, it is difficult to make gel sate if you dissolve 10ml MebiolGel into 20ml culture medium.
Question How many grams of Mebiol gel are there in each container? 
Answer As for 10ml of dilution use, there is 1.0g Mebiol Gel. As for 50ml of dilution use, there is 5.0g Mebiol Gel.
Question As the package is 5g(50ml)/flask, I'd like to aliquot into smaller flasks, how can I deal with it? The question is, we'd like to aliquot it without adding any medium, just aliquot the PNIPAAm-PEG itself. Then we can use different culture medium to dissolve the aliquot ones.
Answer It is desirable that the Mebiol Gel is aliquoted at liquid state and kept in the freezer (-80C). In particular, you should dissolve Mebiol Gel #MBG-PMW20-5005 (5g) with a common solvent (for example, FBS) and aliquote it. Then keep it in a freezer. Please prepare 5X conc. of another component of your desired medium solution and add them to the aliquoted Mebiol Gel at a 4:1 ratio. 

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To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.