Specially modified medium for mice in vitro fertilization, recommended product by CARD (Center of Animal Resources and Development)



The mHTF medium is a novel medium for in vitro fertilization in mice.
It is recommended product by CARD (Center of Animal Resources and Development)

   back to "Mouse Reproductive Engineering" page


mHTF was specially modified for mice in vitro fertilization.

1. For washing or culturing medium for the in-vitro fertilization

2. As a weight for the sperm cryopreservation
   (See Cryopreservation of mouse spermatozoa in FERTIUP®)

3. For washing medium of refrigerated epididymis tailpiece   etc.

The use of CARD MEDIUM®  FERTIUP® will become more and more comfortably with this mHTF.
We supply 2mL and 5mL of mHTF. 5set (2mL) and 3sets (5mL) are also available!

Description Purpose of Use Cat.No. Volume Storage
mHTF    For Mouse in vitro fertilization,
(washing medium,
weight for sperm cryopreservation etc.)
2mL  KYD-008-02-EX     4°C
5*2mL  KYD-008-02-EX-X5
5mL  KYD-008-05-EX
3*5mL  KYD-008-05-EX-X3

We recommend that it is used up at once and please don not store an opened ampule for future usage. Please store it at 4 degrees before use.

Manuals using mHTF

 Cryopreservation of Mouse Spermatozoa
(Using FERTIUP® Cryoprotectant, mHTF, Sperm Straws, Straw connector and Freezing canister)
 In Vitro Fertilization using Cryopreserved Spermatozoa
(Using FERTIUP® Preincubation medium, CARD MEDIUM®, mHTF and Straw connector)
 Collection and Transport at Cold Temperature of Cauda Epididymis(Using Card Cold Transport Kit)
 In Vitro Fertilization using Epidydimal Sperm Transported at Cold Temperature
(Using FERTIUP® Preincubation Medium: PM and mHTF)
  Transport of 2-Cell Embryos at Cold Temperature
(Using Card Cold Transport Kit, M2 and KSOM)
 In vitro fertilization 
(Using FERTIUP® Preincubation medium, CARD MEDIUM® and mHTF)
 Simple Vitrification of Mouse Embryos
(Using DAP213 and DMSO)
 Embryo Transfer into the Oviduct
 Rescue In Vitro Fertilization Method for Legacy Stock of Cryopreserved Spermatozoa
(Using FERTIUP® Preincubation medium: PM, CARD MEDIUM® and mHTF)
 Procedure for Warming of Vitrified Embryos
(Using 0.25M sucrose and KSOM)
 Storage of Media and Solutions in Ampoules Under Nitrogen Gas
 Simple Vitrification of Mouse Oocytes
(Using mHTF)
 Tables of Media Composition
1M DMSO, DAP213, 0.25M Sucrose, mHTF and KSOM

Product List

Product Name Cat# Quantity Price

mHTF ,


2 ML ¥3,000

mHTF ,


5*2 ML ¥12,000

mHTF ,


5 ML ¥6,500

mHTF ,


3*5 ML ¥18,000

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

“mHTF” also belongs to the following categories.