Suitable for HEL (hexanoyl lysine) detection

Anti Hexanoyl-Lys(HEL) monoclonal antibody


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Background

Oxidative damage of lipids caused by reactive oxygen species (ROS) play an important role in some diseases, lesion of cell functions and aging. Aldehydes such as malondi-aldehyde (MDA) and 4-hydroxy-2-nonenal (4-HNE) have been reported as one of the advanced lipid peroxidation products. But recently in the earlier stage of lipid peroxidation, 13-hydroperoxyoctadecanoic acid (13-HPODE) is found to be covalently bound to proteins1). Hexanoyl-Lysine adduct (HEL) is a novel lipid hydroperoxide-modified lysine residues. HEL is formed by oxidative modification by oxidized omega-6 fatty acids such as linoleic acid or arachidonic acid. This monoclonal antibody (clone 5H4)is spesific for proteins and peptides containing HEL. Suitable for HEL detection by immunohistochemistry and western blotting.

A new biomarker for lipid peroxidation:

Application

Immunohistochemistry
Western blotting
ELISA
# This antibody can be applied to tissue samples from human, rabbit, rat and other animals, because lipid oxidation is species independent. But if you are planning to apply to mouse tissue, blocking of internal mouse IgG will be needed before staining. For example, Vector M.O.M. Immunodetection kit (code.PK2200) may be useful for blocking of mouse IgG.

< Immunohistochemistry >
Recommended concentration: 2μg/ml
Application of anti HEL antibody to HUVECsMaeda R, et.al.:Hypertens Res 31(1)p141-151(2008)

Detection of HEL at Human atherosclerotic lesions. Photo: kindly provided by Dr. Naito
Immunohistochemical procedure PDF

< Western blotting >
Multiple bands will be detected because HEL adducts can be formed in any proteins which have lysine residues.
[Reference]:
H. Arai, Y. Kato, K. Fukunaga, S. Mohri, and K. Nakamura: Formation of Nepsilon-(hexanonyl)lysine in oxidized human very-low density lipoprotein. J. Electrophoresis 48, p37-40 (2004)

< Specificity >

< Specificity >
Clone #: 5F12
Subclass: Mouse IgG 1kappa
Immunogen: Hexanoyl modified keyhole limpet hemocyanine
Specificity: Cross reactivity is checked for following loxidized lipidsFMDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonannal, 2-nonenal, 4-hudroxy-2-nonenal
Form: Lyophilized power. Containing 10mM PBS(pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950.100 micro g/mL of IgG.
Storage: Store at less than -20Ž.

<Reference>
1) Yoji Kato, Yoko Mori, Yuko Makino, Yasujiro Morimitsu, Sadayuki Hiroi, Toshitsugu Ishikawa, Toshihiko Osawa, Formation of N epsilon-(hexanonyl) lysine in protein exposed to lipid hydroperoxide. J Biol Chem 274(29), p20406-20414 (1999)
Identification of HEL, which is lysine adduct of 13-HPODE.

2) Kato Y, Miyake Y, Yamamoto K, Shimomura Y, Ochi H, Mori Y, Osawa T, Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle. Biochem Biophys Res Commun 274(2),p389-393(2000)
Development and characterization of anti HEL monoclonal antibody.

3) Fukuchi Y, Miura Y, Nabeno Y, Kato Y, Osawa T, Naito M, Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions. J Atheroscler Thromb 15(4)p185-192(2008)
Application to rabbit atherosclerotic lesions.

4) Maeda R, Noiri E, Isobe H, Homma T, Tanaka T, Negishi K, Doi K, Fujita T, Nakamura E, A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells. Hypertens Res 31(1)p141-151(2008)
Application to cultured cells (HUVECs).

5) Sango K, Yanagisawa H, Kato K, Kato N, Hirooka H, Watabe K: Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells. Open Diabetes J. 1, p1-11(2008)
Application to cultured cell from mouse.

Product List

Product Name Cat# Quantity Price

Anti Hexanoyl-Lys(HEL) (Mouse)  DataSheet

NNS-MHL-021P-EX

20 UG ¥23,100
$308

The clone of anti HEL monoclonal antibody (Cat # : NNS-MHL-020P-EX) will be changed from 5H4 to 5F12 after the end of March 2010.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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