Latest tool to obesity research

Brown Adipocyte Culture Kit


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Background

BAT10 is preadipocyte isolated from rat brown adipose tissue. Adipose tissue plays an important role in mammalian energy equilibrium not only as a lipid-dissipating. White adipose tissue mainly has energy-storing function, but brown adipose tissue has very different function as energy-dissipating due to a unique mitochondrial uncoupling protein (UCP).

Brown adipose tissue is especially abundant in newborns and in hibernating mammals. Its primary function is to generate body heat in animals or newborns that do not shiver. In contrast to white adipocyte, which contain a single lipid droplet, brown adipocyte contain numerous smaller droplets and a much higher number of mitochondria, which contain iron and make it brown. Brown fat also contains more capillaries than white fat, since it has a greater need for oxygen than most tissues


Brown Adipocyte Kit flyer download [PDF]

Features & Usage

Comparion of UCP-1 gene expression between BAT and 3T3-L1
The UCP-1 gene expression of BAT which accumulated intracellular lipid droplets was examined using RT-PCR. The UCP-1 gene expression of BAT increased when stimulated by 1uM norepinephrine.

 

<UCP-1 gene expression in BAT and 3T3-L1 using RT-PCR>


Experiment example

E Search for obesity, diabetic, high blood pressure, and drug for arteriosclerosis
E Development of preventive food of lifestyle-related disease
E Functional test of antiobestic functional food
E Lipid metabolic experiment
E Thermal energy release experiment
E Function clarification of Brown Adipocyte
E Screening of new β3 agonist

References

> Rehnmark S., Kopecky J., Jacobsson A., Nechad M., Herron D., et al. Brown adipocytes differentated in vitro can express the gene for the uncoupling protein thermogenin effects of hypothyroidism and norepinephrine. Exp. Cell Res., (1989) 182:75-83
> G.Ailhaud, P. Grimaldi, and R. Negrel, Cellular and molecular aspects of adipose tissue development. Annu. Rev. Nutr., (1992) 12:207-233
> Yasutake Shimizu and Takashi Shimazu, Effects of wortmannin on increased glucose transport by insulin and norepinephrine in primary culture of brown adipocytes. B.B.R.C., (1994) 202 No.2 July 29 660-665
> Yasutake Shimizu, Danuta Kielar, Yasuhiko Minokoshi and Takashi Shimazu, Noradrenaline increases glucose transport into brown adipocytes in culture by a mechanism different from that of insulin. Biochem. J., (1996) 314:485-490
> Hideki Nikami, Yasutake Shimizu, Michihiro Sumida, Yasuhiko Minokoshi, Toshihide Yosida, Masayuki Saito, and Takashi Shimazu. Expression of ƒÀ3-adrenoceptor and stimulation of glucose transport by ƒÀ3-agonists in brown adipocyte primary culture. J. Biochem. (1996) 119:120-125


Product List

Product Name Cat# Quantity Price

Brown Adipocyte Culture Kit D-i DataSheet ,

PMC-BAT10-COS

1 SET ¥114,000
$1520

Brown Adipocyte Culture kit N-i, Rat

PMC-BAT11-COS

1 SET ¥114,000
$1520

Brown Adipocyte Differentiation Medium DataSheet ,

PMC-BATDM-COS

500 ML ¥31,200
$416

Brown Adipocyte Growth Medium DataSheet ,

PMC-BATGM-COS

500 ML ¥31,200
$416

Brown Adipocyte Maintenance Medium DataSheet ,

PMC-BATMM-COS

500 ML ¥31,200
$416

Brown Adipocyte Culture Medium ,

PMC-BATFM-COS

250 ML ¥31,200
$416
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