Useful for the staining of Tartrate-Resistant Acid Phosphatase in osteoclasts.

TRAP Staining Kit


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Background

The TRAP Staining Kit is used for the staining of Tartrate-Resistant  Acid Phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and the activity of osteoclasts.
Alkaline phosphatase (ALP staining kit, Cat.No.PMC-AK20-COS) and tartrate-resistant acid phosphate are used as markers for osteoblasts and osteoclasts, respectively


Figure 1:@TRAP staining of Osteoclast

Kit component

Component Quantity Storage
Tartrate-containing Buffer 50@mL 4ºC@(Do not freeze)
Chromogenic Substrate 10@vials 4ºC

One kit contains regents for 10 × 96-well plates

#Additional Materials Required
 E10% Formalin Neutral Buffer Solution
 EPhosphate Buffered Saline (PBS)
 EDistilled or deionized water

 

  Frequently Asked Questions

Protocol (96-well plate format)

1. Remove culture medium. Wash each well once with 100uL of PBS.
2. Add 50uL of the 10% Formalin Neutral Buffer Solution to each well and fix for 5 minutes at room temperature.
3. Wash each well with 250uL of deionized water. (× 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5mL of Tartrate-containing Buffer.
5. Add 50uL of Chromogenic Substrates to each well.
6. Incubate at 37 for 20-60 minutes. Adjust incubation time until stained TRAP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.

Note: Excess incubation will be cause of over stained.

 

  Frequently Asked Questions

Product List

Product Name Cat# Quantity Price

TRAP Staining Kit DataSheet ,

PMC-AK04F-COS

10 PLATE ¥33,600
$448