Useful for the staining of alkaline phosphatase in osteoblasts.

Alkaline Phosphatase Staining Kit


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Background

The Alkaline Phosphatase Staining Kit (Cat.No. PMC-AK20-COS) is used for the staining of alkaline phosphatase in osteoblasts. Bone mass is controlled by the balance between the activity of osteoblasts bone formation and the activity of osteoclasts.
Alkaline phosphatase and tartrate-resistant acid phosphate (TRAP : TRAP staining kit , # PMC-AK04F-COS) are used as markers for osteoblasts and osteoclasts, respectively.

  Figure 1:@ ALP staining of 3T3-E1

Kit component

Component Quantity Storage
Substrate-containing Buffer 50 mL 4ºC
Chromogenic Substrate 10 vials 4ºC

One kit contains regents for 10 × 12-well plates

#Additional Materials Required
E10% Formalin Neutral Buffer Solution
EPhosphate Buffered Saline (PBS)
EDistilled or deionized water

Protocol (12-well plate format)

1. Remove culture medium. Wash each well three times with 1mL of PBS.
2. Add 500uL of the 10% Formalin Neutral Buffer Solution to each well and fix for 20 minutes at room temperature
3. Remove 10% Formalin Neutral Buffer Solution. Wash each well with 2mL of deionized water. (× 3 times)
4. Dissolve 1 vial of Chromogenic Substrate with 5mL of Substrate-containing Buffer.
5. Add 400uL of Chromogenic Substrates to each well.
6. Incubate at 37_ for 5-20 minutes. Adjust incubation time until stained ALP is clearly showing the result in figure 1.
7. Wash with deionized water to stop the reaction.

Note: Excess incubation will be cause of over stained

Product List

Product Name Cat# Quantity Price

Alkaline Phosphatase Staining Kit DataSheet ,

PMC-AK20-COS

1 KIT ¥37,000
$494

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.



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