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Useful for analyzing effects of drugs on metabolic syndrome such as obesity, diabetes and hypertension

Epididymal Adipocyte Culture Kit, H-1 (Mouse)


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Background

Epidimal Adipocyte Culture Kit, H-1 (Mouse) (PMC-EAC11-COS) contains preadipocytes isolated from mouse epididymal adipose tissues and culture medium that induces differentiation of precursor cells into mature adipocytes. The kit provides a convenient system for studying the mechanism of adipogenesis as well as for examining effectiveness of drugs on metabolic syndrome such as obesity, diabetes and hypertension.

Kit component

Product Name/Code No. Size Quantity Storage Conditions Shelf Life
Epididymal Preadipocytes, mouse 1.5 x 106 cells 1 Liquid Nitrogen 1 year
Epididymal Adipocyte Culture Medium
(Code: PMC-EACMR-COS)
125 mL 1 -20°C Freezer 6 months
4°C 3 months

Protocol: Culture with 24-well plate

One kit contains preadipocytes for half of a 24-well plate.

1. Thaw Epidimal Adipocyte Culture Medium in a 37°C water bath with gentle shaking.
2. Quickly place Common Precursor Cell vial in a 37°C water bath until the contents are thawed.
3. Transfer thawed cells into a 15 mL centrifuge tube containing 10 mL of Epidimal Adipocyte Culture Medium. Mix gently and centrifuge at 4°C at 170 x g for 5 minutes.
4. Remove the supernatant, and re-suspend cells in 10 mL of Epidimal Adipocyte Culture Medium and centrifuge for 5 minutes at 4°C at 170 x g for 5 minutes.
5. Remove the supernatant, and re-suspend the cell pellet in 6.2 mL of Epidimal Adipocyte Culture Medium.
6. Dispense 0.5 mL of cell suspension to each well of 24-well plate and incubate the plate at 37oC in a 5% CO2 humidified incubator.
7. Gently add 0.5 ml of Epidimal Adipocyte Culture Medium to each well of 24-well plate in the following day.
8. Change the medium every other day.  Be careful to not disturb the cell layer.
    (1) Approximately 3-4 days of culture, preadipocyte culture becomes confluent.
    (2) Approximately 5 days of culture, cells turn to mature adipocytes.
    (3) Approximately 8 days of culture, cells become hypertrophic and start detaching from the bottom of the well.

Cellular Morphology

References

> Yoshida, H., Takamura, N., Shuto, T., Ogata, K., Tokunaga, J., Kawai, K., Kai, H. The Citrus Flavonoids Hesperetin and Naringenin Block the Lipolytic Actions of TNF-a in Mouse Adipocytes. Biochem. Biophys. Res. Commun. 394, 728-732 (2010)

Product List

Product Name Cat# Quantity Price

Epididymal Adipocyte Culture Kit H-1, Mouse DataSheet

PMC-EAC11-COS

1 SET ¥144,000
$1920

Epididymal Adipocyte Culture Medium DataSheet ,

PMC-EACMR-COS

250 ML ¥31,200
$416

Product Name Cat# Quantity Price

Epididymal Adipocyte Culture Kit V-1, Rat DataSheet

PMC-EAC01-COS

1 SET ¥162,000
$2160

Derived from:
SD Rat Epididymal Adipose Tissue

Kit Components:
Epididymal Preadipocytes (Frozen, 1.5 x 106 cells) 1 vial
Epididymal Adipocyte Culture Medium (125 mL) 1 bottle

 

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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