This kit is useful to evaluate osteoclast formation and activation.

Osteoclast Culture Kit


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Background

Osteoclast cells derive from bone marrow precursors. Due to their bone resorption activity, osteoclasts are instrumental in the control of bone mass. When cultured appropriately in the presence of M-CSF and RANKL, bone marrow osteoclast precursors differentiate into osteoblasts displaying bone resorption activity.

The Osteoclast Culture Kits contain bone marrow precursors from rat or mouse, and appropriate wash and growth media to insure osteoclast differentiation. Kits including an Osteo Assay Surface Plate - a 96-well plate coated with a manufactured material that supports osteoclast differentiation and is a substrate for osteoclast resorption activity - allow for the convenient and reproducible observation of osteoclast function.


 Rat Osteoclast

Specifications

Description (Cat. No.) Derived From Kit Components
Osteoclast Culture Kit V-4
(PMC-OSC11-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 4 vials
• Wash Medium (100 ml) 1 bottle
• Culture Medium (50 ml) 1 bottle
Osteoclast Culture Kit V-4
(Osteoassay plate)
(PMC-OSC31-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 4 vials
• Wash Medium (100 ml) 1 bottle
• Culture Medium (50 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well)
Osteoclast Culture Kit V-2
(PMC-OSC12-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
Osteoclast Culture Kit V-2
(Osteoassay plate)
(PMC-OSC32-COS)
SD Rat Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well) 
Osteoclast Culture Kit V-2
(PMC-OSC13-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
Osteoclast Culture Kit V-2 
(PMC-OSC33-COS)
ICR Mouse Bone Marrow

• Osteoclast Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
• Osteo Assay Surface Plate 1 plate (96 well)

Osteoclast Culture Kit V-1
(PMC-OSC14-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle 
Osteoclast Culture Kit V-1 
(PMC-OSC34-COS)
ICR Mouse Bone Marrow • Osteoclast Precursors (Frozen, 2×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle 
• Osteo Assay Surface Plate 1 plate (96 well) 

Osteoclast Culture Kit V-1
(PMC-OSC15-COS)

Human bone marrow • Osteoclast precursor, Human (Frozen, 1.5×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (30 ml) 1 bottle 
Osteoclast Culture Kit V-1
(PMC-OSC35-COS)
Human bone marrow • Osteoclast precursor, Human (Frozen, 1.5×106 cells) 1 vial
• Wash Medium (50 ml) 1 bottle
• Culture Medium (30 ml) 1 bottle 
• Osteo Assay Plate 1 plate (96 well) 

Microscope Images

  Phase contrast microscopy of differentiated osteoclasts on the surface of Osteo Assay Plate.
  TRAP staining of Osteoclasts on the surface of Osteo Assay Plate. TRAP staining kit(Cat No. AK04F) was used.
  Pit on the surface of Osteo Assay Plate (Kossa staining)
References

> Takeshita et al.(2000) Identification and Characterization of the New Osteoclast Progenitor with acrophage Phenotypes Being Able to Differentiate into Mature Osteoclasts. J. Bone Miner. Res. 15, 1477-1488.
> Scheven et al. (1998) A sequential culture approach to study osteoclast differentiation from nonadherent porcine bone marrow cells. In Vitro Cell Dev Biol Anim 34, 568?577.
> Martha et al. (1995) Enhanced Expression of ƒ¿V Integrin Subunit and Osteopontin during Differentiation of HL-60 Cells along the Monocytic Pathway. Exp. Cell Res. 216, 335-341.
> Itonaga et al.(1999) 1,25-Dihydroxyvitamin D3 and Prostaglandin E2 Act Directly on Circulating Human Osteoclast Precursors. Biochem. Biophys. Res. Commun. 242, 703-709.

Product List

Product Name Cat# Quantity Price

Osteoclast Culture Kit V-4 DataSheet ,

PMC-OSC11-COS

1 SET ¥152,400
$2032

Osteoclast Culture kit V-4 (Osteoassay plate) DataSheet ,

PMC-OSC31-COS

1 SET ¥176,400
$2352

Osteoclast Culture Kit V-2 DataSheet ,

PMC-OSC12-COS

1 SET ¥88,800
$1184

Osteoclast Culture kit V-2 (Osteoassay plate) ,

PMC-OSC32-COS

1 SET ¥112,800
$1504

Osteoclast Culture Kit V-2 DataSheet ,

PMC-OSC13-COS

1 SET ¥238,800
$3184

Osteoclast Culture Kit V-2, Mouse

PMC-OSC33-COS

1 SET ¥288,000
$3840

Osteoclast Culture Kit V-1 DataSheet ,

PMC-OSC14-COS

1 SET ¥120,000
$1600

Osteoclast Culture Kit V-1, Mouse

PMC-OSC34-COS

1 SET ¥156,000
$2080

Osteoclast Culture Kit V-1, Human DataSheet

PMC-OSC15-COS

1 SET ¥198,000
$2640

Osteoclast Culture Kit V-1 (Osteoassay plate), Human DataSheet

PMC-OSC35-COS

1 SET ¥222,000
$2960

Osteoclast Culture Medium (for Rat) DataSheet ,

PMC-OSCMR-COS

50 ML ¥36,000
$480

Osteoclast Culture Medium (for Mouse) DataSheet ,

PMC-OSCMM-COS

50 ML ¥36,000
$480

Osteoclast Wash Medium DataSheet ,

PMC-OSCMW-COS

100 ML ¥13,200
$176

RANKL (Human), Recombinant ,

PMC-AK30-COS

10 UG ¥21,600
$288

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


“Osteoclast Culture Kit” also belongs to the following categories.