Monocyte Culture Kit


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Background

Monocytes are produced by the differentiation of monocyte precursor cell in bone marrow. In tissue, monocytes mature into macrophages and are involved in immunity, recovering tissues and etc.

Spedifications

Description (Cat. No.) Derived From Kit Components
Monocyte Culture Kit V-2
(PMC-BMM01-COS)
SD Rat Bone Marrow • Common Precursors (Frozen, 2×106 cells) 2 vials
• Wash Medium (50 ml) 1 bottle
• Culture Medium (25 ml) 1 bottle
Monocyte Culture Kit V-1
(PMC-BMMC-COS)
SD Rat Bone Marrow • Common Precursors (Frozen, 2×106 cells) 1 vial

Precautions

1. Read the instructions carefully before beginning the culture.
2. This kit is for research use only, not for human or diagnostic use.
3. Upon receiving, cryovials are immediately transferred to and stored in liquid nitrogen until ready to use. It is recommended to use Monocyte Precursor cell vial immediately upon receiving.

Protocol

1. Thaw Wash Medium and Culture Medium in a 37°C water bath with gentle shaking.
2. Quickly place Monocyte Precursor Cell vial in a 37°C water bath until its content is completely thawed.
3. Transfer thawed cells into a 15 ml centrifuge tube containing 10 ml of Wash Medium and centrifuge at 4°C at 400x g for 5 minutes to pellet the cells.
4. Remove the supernatant, and re-suspend cells in 10 ml of Wash Medium and centrifuge at 4°C at 400x g for 5 minutes.
5. Remove the supernatant, and re-suspend the cell pellet in a total volume of 5 ml of Culture Medium.
6. Transfer the cell suspension to culture plate (5ml/6cm dish or 1ml/well/24well-plate) and incubate at 37°C in a 5% CO2, humidified incubator.

* Low cell binding culture plates such as HydroCell (CellSeed Inc.), are recommended to culture monocytes as the cells are capable of strongly adhering to normal culture plates.
* The size of cells is about several micrometers in the first cell culture and may reach 10 micrometers in days 3-4 days of culture (Figure 1). When cells are cultured for a period of time they are able to form several tens of colonies.

Phase Contrast Anti-Mac1 FITC Staining
Figure 1
References

> Sunao Takesita, Keisuke Kaji, Akira Kudo. Identification and Characterization of the New Osteoclast Progenitor with Macrophage Phenotypes Being Able to Differentiate into Mature Osteoclasts. JOURNAL OF BONE AND MINERAL RESEARCH Volume 15, Number 8 (2000) .1477-1488

Product List

Product Name Cat# Quantity Price

Monocyte Culture Kit V-2 DataSheet ,

PMC-BMM01-COS

1 SET ¥112,800
$1504

Monocyte Precusor Cell V-1, Rat DataSheet

PMC-BMMC-COS

1 VIAL ¥48,000
$640

Monocyte Culture Medium DataSheet ,

PMC-BMMG-COS

25 ML ¥24,000
$320

Monocyte Wash Medium DataSheet ,

PMC-BMMW-COS

50 ML ¥24,000
$320

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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