useful for experiments as a bate to identify the protein-protein interaction in vivo

Anti-LexA polyclonal antibody


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Background

E. coli LexA protein binds specifically to the SOS-box sequencejand represses the genes belonging to the SOS regulon. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As the results, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1).
The lexA gene is used for yeast two-hybrid experiments as a bate to identify the protein-protein interaction in vivo (2).
This product was prepared by immunizing rabbit with full-size highly-purified recombinant LexA protein. Using this antibody, 23 kD LexA protein was identified in the E.coli whole-cell lysate (Fig 1) and the expression of bait constructs was identified in yeast extracts by western blotting.

 

Fig.1 Detection of LexA repressor in the whole cell lysate of E. coli by@this antiserum

1)Studies on the SOS regulation in E.coli (3). For western blotting; 1000~3000 fold dilution.

2)Construction and expression of a bait protein fused to LexA protein can be examined by western blotting of the yeast extracts,using the antiserum.
Purified LexA protein (Cat#BAM-01-005, Cat#BAM-01-006) is available to be used as a positive control for western blotting.

 

 

<Reference>
1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd Ed., ASM Presss (2005)
2. Sambrook J & Russell DW, Molecular Cloning 3rd Ed. Cold Spring Harbor Press (2001)
3. Hishida T, et al., Genes Dev.18, 1886-1897 (2004)

Product List

Anti LexA, BAM-61-001, Species : _III_ Escherichia coli_IIII_ , Host : RAB, Application : WB
Anti LexA, BAM-61-002, Species : _III_ Escherichia coli_IIII_ , Host : RAB, Application : WB


Product Name Cat# Quantity Price

Anti LexA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-001-EX

50 UL ¥21,000
$280

Anti LexA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-002-EX

250 UL ¥84,000
$1120

Purified LexA protein


Product Name Cat# Quantity Price

LexA repressor Escherichia coli DataSheet

BAM-01-005-EX

20 UG ¥42,000
$560

LexA repressor Escherichia coli DataSheet

BAM-01-006-EX

100 UG ¥126,000
$1680

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