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Anti Rad22 (fission yeast : S.pombe) polyclonal antibody


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Background

Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S. cerevisiae and human Rad22 proteins, which play a major role in genetic recombination and recombination repair by mediating strand annealing reaction between homologous DNA strands (1).
The product was prepared by immunizing rabbit with recombinant Rhp22 protein expressed in E. coli and purified.
Using this anti-serum, Rad22 protein (52 kD) in the crude extract was detected by Western blotting (Fig 1)

Application

  1. Western blotting (x 2,000~5,000 dilution)
  2. Immuno-precipitation
  3. Detection of foci formation by indirect@immunofluorescence

Fig.1 Western blot analysis of Rad22 in whole cell extracts.
   M       FMolecular weight markers (kD)
   Lane 1FWild-type strain.
   Lane 2Frad22 deletion strain.

<Reference>
1. Lehmann AR, Mutation Res. 363, 147 (1996) Review

Product List

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