High affinities IgE receptor antibody

Anti 5-Methylcytosine antibody


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Background

DNA methylation is a type of chemical modification of DNA that can be inherited and subsequently removed without changing the original DNA sequence. As such, it is part of the epigenetic code and is also the most well characterized epigenetic mechanism. DNA methylation involves the addition of a methyl group to DNA — for example, to the number 5 carbon of the cytosine pyrimidine ring — in this case with the specific effect of reducing gene expression. In adult somatic tissues, DNA methylation typically occurs in a CpG dinucleotide context; non-CpG methylation is prevalent in embryonic stem cells. This hybridoma has been prepared by Prof. H. Sano of NARA INSTITUTE of SCIENCE and TECHNOLOGY

Application

1) Immunocytochemistry (Figure below and Ref.1 & 2) (~50-100 fold dilution)
2) Immuno-blotting detection of DNA with 5-methylocytosine on nitrocellulose (Ref. 3 & 4) (~1000 fold dilution).

Methylation of chloroplast DNA visualized by immunochemistry.
Samples are Chlamidomonas me-1 cells. Left: DAPI-stained cells. Middle: Cells stained with anti-5MeC antibody and FITC-conjugated 2nd antibody. Right: Merged image. Chloroplast DNA is exclusively methylated in gamete cells

<Reference>
1. Sharif J. et al. The SRA protein Np95 mediates epigenetic inheritance by recruiting Dmnt1 to methylated DNA. Nature 450: 908-912 (2007)
2. Nishiyama R. et al. A chloroplast-resident DNA methyltransferase is responsible for hypermethylation of chloroplast genes in Chlamydomonas maternal gametes.@PNAS 99:@5925-30 (2002).
3. Sano H, Sager R. Detection of heavy methylation in human repetitive DNA subsets by a monoclonal antibody against 5-methylcytosine Biochim Biophys Acta. 951:157-65 (1988).
4. Sano H. Royer HD. & Sager R. Identification of 5-methycytosine in DNA fragment immobilized on nitrocellulose paper. PNAS 77:3581-85 (1980) 

Product List

Product Name Cat# Quantity Price

Anti 5-Methylcytosine (Mouse)  DataSheet

BAM-51-001-EX

100 UL N/A

Anti 5-Methylcytosine (Mouse)  DataSheet

BAM-51-003-EX

100 UG ¥34,500
$460

Product Name Cat# Quantity Price

Anti Swi6 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-101-EX

50 UL ¥30,000
$400

Anti Swi6 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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250 UL N/A

Anti Cut5/Rad4 Saccharomyces cerevisiae  (Rabbit)  DataSheet

BAM-63-107-EX

100 UL ¥35,000
$467

Anti Dis1 Schizosaccharomyces pombe  (Rabbit) Unlabeled

BAM-63-117-EX

200 UL contact us

Anti DIS3 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥30,000
$400

Anti H2B Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-125-EX

50 UL ¥30,000
$400

Anti Pad Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-133-EX

100 UL ¥35,000
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Anti Ppa2 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-135-EX

100 UL ¥35,000
$467

Anti Psm1 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-137-EX

100 UL ¥35,000
$467

Anti Sds22 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-63-141-EX

100 UL ¥35,000
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Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

BAM-63-151-EX

50 UL ¥30,000
$400

Anti Mrc1 Schizosaccharomyces pombe  (Rabbit)  DataSheet

BAM-63-153-EX

250 UL N/A

Product Name Cat# Quantity Price

Anti Dnmt1 (1-248), Mouse (Rabbit) Unlabeled DataSheet

BAM-70-201-EX

50 UG ¥26,300
$351

Anti Dnmt1 (1037-1386), Mouse (Rabbit) Unlabeled DataSheet

BAM-70-203-EX

50 UG ¥29,000
$387

Anti HP1 (α) /CBX5, Human (Rabbit)  DataSheet

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50 UG ¥21,000
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Anti HP1 (β)/CBX1, Human (Rabbit)  DataSheet

BAM-70-223-EX

50 UG ¥21,000
$280

Anti HP1 (γ)/CBX3, Human (Rabbit)  DataSheet

BAM-70-225-EX

50 UG ¥21,000
$280

Anti 5-Methylcytosine (Mouse)  DataSheet

BAM-51-001-EX

100 UL N/A

Anti 5-Methylcytosine (Mouse)  DataSheet

BAM-51-003-EX

100 UG ¥34,500
$460

Product Name Cat# Quantity Price

Anti Rpn3 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-201-EX

100 UL ¥40,000
$534

Anti Rpn5 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-203-EX

100 UL ¥40,000
$534

Anti Rpn7 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

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100 UL ¥40,000
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Anti Rpn9 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-207-EX

100 UL ¥42,000
$560

Anti Rpn12 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-209EX

50 UL ¥30,000
$400

Anti Nob1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-211-EX

100 UL ¥42,000
$560

Anti Nas6/p28 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-213-EX

100 UL ¥42,000
$560

Anti Tem1 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-215-EX

100 UL ¥42,000
$560



“Anti 5-Methylcytosine antibody” also belongs to the following categories.