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Anti SLA (haploid sperm cell-specific antigen) monoclonal antibody [clone : TRA54]


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Background

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organellaes in germ cell cytoplasm from spermatocytes to spermatids (1, 2). Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis (fig WB). This antibody can be used for immunohistochemical staining of testicular tissues(1, 3).
The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by Prof. Yoshitake Nishimune group of Osaka University. This product is purified from the ascitic fluid of nude mice carrying the hybridoma clone TRA54.

Kit component

1) Western blotting
2) Immunohistochemical staining


Western blot analysis of various mouse tissues with TRA54 antibody. The antibody was 5,000-fold diluted before use.

<Reference>
1. Pereira LAVD, et al.@Int. J Androl. 21, 34-40 (1998)
2. Arrote Anti-SLA (haploid sperm cell-specific antigen)ia KF, et al.,
Androl. 25, 914-921 (2004)
3. Ventela S, et al. Mol. Biol. Cell. 14, 2768-2780 (2003)

Product List

Product Name Cat# Quantity Price

Anti SLA, Mouse (Rat) Unlabeled DataSheet

BAM-73-001-EX

50 UG see also
# BAM-73001EX
Size 100

Anti SLA, Mouse (Mouse) Unlabeled DataSheet

BAM-73-002-EX

250 UG N/A

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