• HOME
  • Products
  • Anti Rad51p(human) polyclonal antibody (IgY affinity purified)

Anti Rad51p(human) polyclonal antibody (IgY affinity purified)


print

Background

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs, Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogenes and tumor suppressors such as BRACA1, BRACA2, and P53 for the maintenance of genome stability (1). The product was purified (Fig.1) from the eggs of immunized chicken with full-size recombinant Rap51 protein expressed in E. coli and affinity purified. Affinity purification increased antibody titer 30-fold.
Using this anti-serum, Rad51 protein (37 kD) in the crude extract was detected by Western blotting (Fig 2) at 40 kD position and Rad51 foci formation induced by stalled replication and DNA-damage (2) was detected by indirect immunofluorescence. This antibody was successfully used for immunoprecipitatio , western blot and foci detection.

Application

1) Western blotting (10000~50000 dilution)
2) Immuno-precipitation
3) Detection of foci formation by indirect immunofluorescence


Fig.1 SDS-PAGE (without Et-SH) analysis of purified IgY

Fig.2 Western blot analysis of Rap51 in HeLa cell extract.

 

<Reference>
1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd ed., ASM Press
2. Tashiro S, et al., Rad51 accumulation at sites of DNA damage and in postreplicative chromatin. J Cell Biol 150; 283-291 (2000) 

Product List

Product Name Cat# Quantity Price

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-009EX

50 UG N/A

Related Products


Product Name Cat# Quantity Price

Anti CPDs (Mouse)  DataSheet

CAC-NM-DND-001

1 VIAL ¥44,000
$587

Anti 6-4PPs (Mouse)  DataSheet

CAC-NM-DND-002

1 VIAL ¥44,000
$587

Anti DewarPPs, Human (Mouse)  DataSheet

CAC-NM-DND-003

1 VIAL ¥44,000
$587

Product Name Cat# Quantity Price

Anti LexA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-001-EX

50 UL ¥21,000
$280

Anti LexA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-002-EX

250 UL ¥84,000
$1120

Anti RecA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-003-EX

50 UG ¥31,500
$420

Anti RecA Escherichia coli  (Rabbit) Unlabeled DataSheet

BAM-61-004-EX

250 UG ¥124,500
$1660

Anti XPA, Human (Mouse) Unlabeled DataSheet

BAM-70-031-EX

50 UG see also
# BAM-70031EX
Size 100

Anti XPA, Human (Mouse) Unlabeled DataSheet

BAM-70-032-EX

250 UG N/A

Anti DNA polymerase (β), Rat (Rabbit) Unlabeled DataSheet

BAM-70-041-EX

50 UL see also
# BAM-70041EX
Size 100

Anti DNA polymerase (β), Rat (Rabbit) Unlabeled DataSheet

BAM-70-042-EX

250 UL N/A

Anti DNA polymerase (Δ) subunit p66, Human (Mouse) Unlabeled DataSheet

BAM-70-055EX

20 UG N/A

Anti DNA polymerase (Δ) subunit p66, Human (Mouse) Unlabeled DataSheet

BAM-70-056EX

100 UG ¥34,800
$464

Product Name Cat# Quantity Price

Anti Rad18, Human (Rabbit) Unlabeled DataSheet

BAM-70-023-EX

100 UG ¥34,500
$460

Anti Rad22/Rad52 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-003-EX

50 UG ¥34,500
$460

Anti Rhp51 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-001-EX

50 UL ¥30,000
$400

Anti Rhp51 Schizosaccharomyces pombe  (Rabbit) Unlabeled DataSheet

BAM-63-002-EX

250 UL N/A

Anti Rad51 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-101-EX

50 UL ¥31,600
$422

Anti Rad51 Saccharomyces cerevisiae  (Rabbit) Unlabeled DataSheet

BAM-62-102-EX

250 UL N/A

Anti Rad51, Human (Rabbit) Unlabeled DataSheet

BAM-70-001-EX

50 UL ¥24,500
$327

Anti Rad51, Human (Rabbit) Unlabeled DataSheet

BAM-70-002-EX

250 UL ¥73,500
$980

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-003-EX

50 UL see also
# BAM-70003EX
Size 100

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-004-EX

250 UL N/A

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-005-EX

100 UG ¥33,000
$440

Anti Rad51, Human (Chicken) Agarose DataSheet

BAM-70-007-EX

100 UL ¥31,600
$422

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-009EX

50 UG N/A

Anti Rad51, Human (Chicken) Unlabeled DataSheet

BAM-70-010-EX

50 UG N/A

Anti Rhp55/Rad55 Schizosaccharomyces pombe  (Rabbit) Unlabeled

BAM-63-005-EX

50 UL N/A

Anti Rhp57/Rad57 Schizosaccharomyces pombe  (Rabbit) Unlabeled

BAM-63-007-EX

50 UL N/A

Anti Rad60 Schizosaccharomyces pombe  (Rabbit) Unlabeled

BAM-63-009-EX

50 UL contact us

“Anti Rad51p(human) polyclonal antibody (IgY affinity purified)” also belongs to the following categories.