Anti-Rad18 (human) polyclonal antibody


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Background

The Rad6-Rad18 pair of genes plays a critical role in post-replication repair of damaged DNA. Rad6 protein functions as an E2 enzyme and Rad18 as a ubiquitine ligase (E3) which ubiquitinates PCNA. Rad18 recruits translesion DNA polymerases to damaged DNA (ref 1~3).

Application

1) Western blotting (1,000 fold dilution)
2) Indirect immuno-fluorescence staining
3) Immuno-precipitation


Fig 1.Indirect immuno-fluorescence staining of Rad18 protein in GM637 cells.
Rad18 protein is stained as yellow dots in nuclei.


Fig 2.Identification of Rad18 protein in crude extract of A549 cells by western blotting.
The lower thick band is native Rad18 and the upper thin band is mono-ubiquitinated Rad18 protein (ref. 1)

<Reference>
1. Miyase S. et al.J. Biol. Chem. 280, 515-524 (2005)
2. Tateishi S. et al.PNAS 97, 7927-7932 (2000)
3. Watanabe K. et al. EMBO J. 23, 3886-3896 (2004)

Product List

Product Name Cat# Quantity Price

Anti Rad18, Human (Rabbit) Unlabeled DataSheet

BAM-70-023-EX

100 UG ¥34,500
$460

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