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Anti Rpn5 (S.cerevisiae) polyclonal antibody


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Background

The 26 S proteasome is a protein complex with a molecular mass of 2000 kDa. It is essential not only for eliminating damaged or misfolded proteins but also for degrading short lived regulatory proteins involved in cell cycle regulation, DNA repair, signal transduction, apoptosis, and metabolic regulation (1). Rpn5p is essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7 ( 2, 3,). It consists of 445 amino acids with molecular weight of 51,768.

Application

1) Western blotting (-1000 fold dilution)
2) Immunoprecipitation
Not tested for other applications

Detection of Rpn5 (52 kDa) in the crude extract of S. cerevisiae by Western blotting using this antibody.

<Reference>
This product was used in ref.3
1. Hershko, A., and Ciechanover, A. THE UBIQUITIN SYSTEM Annu. Rev. Biochem. 67, 425-479 (1998)
2. Finley D, et al. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle.Trends Biochem Sci 23:244 (1998)
3. Isono E, et al. The assembly pathway of the 19S regulatory particle of the yeast 26S proteasome. Mol. Biol. Cell 18 : 569 (2007)

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